Team:Brown-Stanford/Lab/Protocols/Bang

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*1% tryptone
*1% tryptone

Revision as of 18:57, 13 July 2011

Brown-Stanford
iGEM

Protocol Code: M7

Bang Media

By Percentage

  • 1% tryptone
  • 0.5% yeast extract
  • 0.45 tricine
  • 0.5% (NH4)2SO4
  • 0.2% glutamic acid
  • 1% urea

By Mass per Liter =

  • 10g tryptone
  • 5g yeast extract
  • 4.5g tricine
  • 5g (NH4)2SO4
  • 2g glutamic acid
  • 10g urea
  • Optional: 15g agar (for plates)

Mix in 200mL of water (without agar) to make a 5x solution.