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Team:Fatih Turkey/Experiment3C
From 2011.igem.org
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<td width="600" height="351" valign="top"><p><strong>Inhibition in Liquid Culture Assay</strong><br /> | <td width="600" height="351" valign="top"><p><strong>Inhibition in Liquid Culture Assay</strong><br /> | ||
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| + | <p> We wanted to see the effects of supernatant of B.subtilis which produces LALF in order to stop growth of E.coli with RFP in liquid culture.<br /> | ||
We added 8 ml LB broth in falcons and determined 3 control groups. These were: </p> | We added 8 ml LB broth in falcons and determined 3 control groups. These were: </p> | ||
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Latest revision as of 20:04, 28 October 2011
Inhibition in Liquid Culture Assay
We wanted to see the effects of supernatant of B.subtilis which produces LALF in order to stop growth of E.coli with RFP in liquid culture.
First of all, we put 5 ul E.coli which has RFP into 8 falcons as experimental groups. We waited experimental falcons for 12 hours in 37⁰C. Then, we added 1 ul, 10 ul, 100 ul and 1000 ul liquid culture with LALF and liquid culture without LALF. After this, we incubated them in 37⁰C and measured their OD value after 2 - 4 - 8 - 12 hours in 405 nm. You can see the results below: |
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