We wanted to see the effects of supernatant of B.subtilis which produces LALF in order to stop growth of E.coli with RFP in liquid culture.
We added 8 ml LB broth in falcons and determined 3 control groups. These were:
Control group 1: Includes only 5 ul E.coli which waited for 12 hours in 37⁰C. These can synthesize RFP.
Control group 2: Includes only 5 ul B.subtilis which produces LALF. They waited for 12 hours in 37⁰C.
Control group 3: Includes only 5 ul B.subtilis which do not produce LALF. They waited for 12 hours in 37⁰C.
First of all, we put 5 ul E.coli which has RFP into 8 falcons as experimental groups. We waited experimental falcons for 12 hours in 37⁰C. Then, we added 1 ul, 10 ul, 100 ul and 1000 ul liquid culture with LALF and liquid culture without LALF. After this, we incubated them in 37⁰C and measured their OD value after 2 - 4 - 8 - 12 hours in 405 nm. You can see the results below: