Team:Brown-Stanford/Lab/Protocol/BiobrickRestrictionDigest

From 2011.igem.org

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(Created page with "=='''Biobrick Restriction Digest'''== (Taken from [http://openwetware.org/wiki/Restriction_digest openwetware.org]; the protocol was subsequently edited for restriction of [http...")
 
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{{:Team:Brown-Stanford/Templates/Protocol}}
=='''Biobrick Restriction Digest'''==
=='''Biobrick Restriction Digest'''==
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(Taken from [http://openwetware.org/wiki/Restriction_digest openwetware.org]; the protocol was subsequently edited for restriction of [http://partsregistry.org/Part:BBa_K656013 Part BBa_K656013])
+
(Taken from [http://openwetware.org/wiki/Restriction_digest openwetware.org]; the protocol was subsequently edited for restriction of [http://partsregistry.org/Part:BBa_K656013 Part BBa_K656013] and [http://partsregistry.org/Part:pSB1C3 pSB1C3])
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'''Reagents for BBa_K656013 digestion'''
 +
* 4.6 µL nuclease-free water
 +
* 27 µL purified PCR product
 +
* 4 µL appropriate NEBuffer
 +
* .4 µL BSA
 +
* 2 µL each restriction enzyme
 +
 
 +
'''Treatment for BBa_K656013'''
 +
# Combine all reagents in a single PCR tube
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# Incubate at 37˚C for 3 hours
 +
 
 +
'''Reagents for pSCB1C3 digestion'''
 +
* 27.4 µL nuclease-free water
 +
* 3.6 µL miniprepped DNA sample
 +
* 4 µL appropriate NEBuffer
 +
* .4 µL BSA
 +
* 1 µL each restriction enzyme
 +
 
 +
'''Treatment for pSCB1C3"
 +
# Combine all reagents in a single PCR tube
 +
#Incubate at 37˚ for 1 hour

Latest revision as of 18:19, 28 September 2011

Brown-Stanford
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Biobrick Restriction Digest

(Taken from [http://openwetware.org/wiki/Restriction_digest openwetware.org]; the protocol was subsequently edited for restriction of [http://partsregistry.org/Part:BBa_K656013 Part BBa_K656013] and [http://partsregistry.org/Part:pSB1C3 pSB1C3])

Reagents for BBa_K656013 digestion

  • 4.6 µL nuclease-free water
  • 27 µL purified PCR product
  • 4 µL appropriate NEBuffer
  • .4 µL BSA
  • 2 µL each restriction enzyme

Treatment for BBa_K656013

  1. Combine all reagents in a single PCR tube
  2. Incubate at 37˚C for 3 hours

Reagents for pSCB1C3 digestion

  • 27.4 µL nuclease-free water
  • 3.6 µL miniprepped DNA sample
  • 4 µL appropriate NEBuffer
  • .4 µL BSA
  • 1 µL each restriction enzyme

Treatment for pSCB1C3"

  1. Combine all reagents in a single PCR tube
  2. Incubate at 37˚ for 1 hour
Retrieved from "http://2011.igem.org/Team:Brown-Stanford/Lab/Protocol/BiobrickRestrictionDigest"