Team:Brown-Stanford/Lab/Protocols/DM3 plates

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== '''DM3 Plates''' ==
== '''DM3 Plates''' ==
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=== '''Notes''' ===
=== '''Notes''' ===
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*Autoclave everything except BSA
*Filter sterilize BSA and add when mixture is ~55˚C
*Filter sterilize BSA and add when mixture is ~55˚C
{{:Team:Brown-Stanford/Templates/Foot}}
{{:Team:Brown-Stanford/Templates/Foot}}

Latest revision as of 18:18, 28 September 2011

Brown-Stanford
iGEM

Back to
iGEM HQ

DM3 Plates

DM3 plates:

  • Agar 200ml 4%
  • Sodium Succinate 500ml 1M pH 7.3
  • Casamino Acids 100ml 5%
  • Yeast Extract 50ml 10%
  • K2HPO4 3.5% + KH2PO4 1.5% in 100ml
  • Glucose 15ml 20%
  • MgCl2 20ml 1M
  • BSA 5ml 2%

Notes

  • Autoclave everything except BSA
  • Filter sterilize BSA and add when mixture is ~55˚C

Retrieved from "http://2011.igem.org/Team:Brown-Stanford/Lab/Protocols/DM3_plates"