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Team:Brown-Stanford/Lab/Protocols/DM3 plates
From 2011.igem.org
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| - | {{:Team:Brown-Stanford/Templates/ | + | {{:Team:Brown-Stanford/Templates/Protocol}} |
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== '''DM3 Plates''' == | == '''DM3 Plates''' == | ||
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=== '''Notes''' === | === '''Notes''' === | ||
| + | *Autoclave everything except BSA | ||
*Filter sterilize BSA and add when mixture is ~55˚C | *Filter sterilize BSA and add when mixture is ~55˚C | ||
{{:Team:Brown-Stanford/Templates/Foot}} | {{:Team:Brown-Stanford/Templates/Foot}} | ||
Latest revision as of 18:18, 28 September 2011
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REGObricks
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DM3 Plates
DM3 plates:
- Agar 200ml 4%
- Sodium Succinate 500ml 1M pH 7.3
- Casamino Acids 100ml 5%
- Yeast Extract 50ml 10%
- K2HPO4 3.5% + KH2PO4 1.5% in 100ml
- Glucose 15ml 20%
- MgCl2 20ml 1M
- BSA 5ml 2%
Notes
- Autoclave everything except BSA
- Filter sterilize BSA and add when mixture is ~55˚C
