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Team:Amsterdam/Notebook/Protocols/Transforming electrocompetent Cells
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| - | + | We use a protocol commonly used in our host lab provided by [https://2011.igem.org/Team:Amsterdam/Team/Advisors Lisette Anink]. The main advantage of electrocompetent cells compared to chemically competent cells is a higher level of competence (1-2 log higher). The disadvantage, though, is the high price of electroporation cuvettes and the implications this has on maximum number of transformations that can be performed in a single experiment. | |
===Materials=== | ===Materials=== | ||
Revision as of 09:49, 21 September 2011
Contents |
Transforming electrocompetent cells
Overview
We use a protocol commonly used in our host lab provided by Lisette Anink. The main advantage of electrocompetent cells compared to chemically competent cells is a higher level of competence (1-2 log higher). The disadvantage, though, is the high price of electroporation cuvettes and the implications this has on maximum number of transformations that can be performed in a single experiment.
Materials
Procedure
