Team:Amsterdam/9 September 2011
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==Constructs== | ==Constructs== | ||
+ | An ON ligation was performed at 16°C to make the following constructs: | ||
+ | *K538130-Cpn60 | ||
+ | *K206000-K538101 | ||
==Backbone transferring== | ==Backbone transferring== | ||
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*The constructs we got thus far | *The constructs we got thus far | ||
+ | ===Gel purification=== | ||
+ | The different parts were put on gel and purified with the qiaquick gel extraction kit. | ||
+ | The following parts showed a good result: | ||
+ | *Cpn10 | ||
+ | *K538104 | ||
+ | *CspC | ||
+ | *K538101 | ||
+ | *Cpn60 | ||
- | + | ===Ligation=== | |
- | + | An ON ligation was performed at 16°C to transfer the following parts (from 08-09) into the Chloramphenicol backbone: | |
- | + | *K538101 | |
- | + | *Cpn60 | |
- | + | *K538120 | |
- | + | ||
- | + | ||
{{:Team:Amsterdam/Footer}} | {{:Team:Amsterdam/Footer}} |
Revision as of 08:43, 21 September 2011
![](https://static.igem.org/mediawiki/2011/4/47/Ams11_bg.jpg)
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09-09-2011
Constructs
An ON ligation was performed at 16°C to make the following constructs:
- K538130-Cpn60
- K206000-K538101
Backbone transferring
Digestion of different parts to tranfer to the Chloramphenicol backbone:
- All genes
- The constructs we got thus far
Gel purification
The different parts were put on gel and purified with the qiaquick gel extraction kit. The following parts showed a good result:
- Cpn10
- K538104
- CspC
- K538101
- Cpn60
Ligation
An ON ligation was performed at 16°C to transfer the following parts (from 08-09) into the Chloramphenicol backbone:
- K538101
- Cpn60
- K538120