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Team:Brown-Stanford/Lab/Protocols/BG11
From 2011.igem.org
(Difference between revisions)
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=== '''Stock solutions for BG-11''' === | === '''Stock solutions for BG-11''' === | ||
Stock 1: | Stock 1: | ||
| - | 0.1g/liter Na<sub>2</sub>MG EDTA | + | *0.1g/liter Na<sub>2</sub>MG EDTA |
| - | 0.6g/liter Ferric ammonium citrate | + | *0.6g/liter Ferric ammonium citrate |
| - | Citric acid•1H<sub>2</sub>O | + | *0.6g/liter Citric acid•1H<sub>2</sub>O |
| - | + | *3.6g/liter CaCl<sub>2</sub>•2H<sub>2</sub>O | |
| - | CaCl<sub>2</sub>•2H<sub>2</sub>O | + | Filter sterilize into a sterile bottle or autoclave. |
| - | + | ||
| - | Filter sterilize into a sterile bottle or autoclave | + | |
Stock 2: | Stock 2: | ||
| - | MgSO<sub>4</sub>•7H<sub>2</sub>O | + | *7.5g/liter MgSO<sub>4</sub>•7H<sub>2</sub>O |
| - | + | Filter sterilize into a sterile bottle or autoclave. | |
| - | Filter sterilize into a sterile bottle or autoclave | + | |
Stock 3: | Stock 3: | ||
| - | K<sub>2HPO</sub>4•3H<sub>2</sub>O | + | *4.0g/liter K<sub>2HPO</sub>4•3H<sub>2</sub>O |
| - | + | *or 3.05g/liter K<sub>2</sub>HPO<sub>4</sub> | |
| - | + | Filter sterilize into a sterile bottle or autoclave. | |
| - | + | ||
| - | Filter sterilize into a sterile bottle or autoclave | + | |
Stock 5 (Microelements): | Stock 5 (Microelements): | ||
| - | + | *2.86g/liter H<sub>3</sub>BO<sub>3</sub> | |
| - | 2.86g/liter | + | *1.81g/liter MnCl<sub>2</sub>•4H<sub>2</sub>O |
| - | MnCl<sub>2</sub>•4H<sub>2</sub>O | + | *0.222g/liter ZnSO<sub>4</sub>•7H<sub>2</sub>O |
| - | + | *0.079g/liter CuSO<sub>4</sub>•5H<sub>2</sub>O | |
| - | ZnSO<sub>4</sub>•7H<sub>2</sub>O | + | *0.050g/liter COCl<sub>2</sub>•6H<sub>2</sub>O |
| - | 0. | + | *0.391g/liter NaMoO<sub>4</sub>•2H<sub>2</sub>O |
| - | CuSO<sub>4</sub>•5H<sub>2</sub>O | + | *or 0.018g/liter MoO<sub>4</sub> (85%) |
| - | 0. | + | |
| - | COCl<sub>2</sub>•6H<sub>2</sub>O | + | |
| - | 0. | + | |
| - | NaMoO<sub>4</sub>•2H<sub>2</sub>O | + | |
| - | 0. | + | |
| - | + | ||
| - | + | ||
Revision as of 23:37, 11 June 2011
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BG-11
(Taken from [http://microbiology.ucdavis.edu/meeks/BG11medium.html UC Davis])
Stock solutions for BG-11
Stock 1:
- 0.1g/liter Na2MG EDTA
- 0.6g/liter Ferric ammonium citrate
- 0.6g/liter Citric acid•1H2O
- 3.6g/liter CaCl2•2H2O
Filter sterilize into a sterile bottle or autoclave.
Stock 2:
- 7.5g/liter MgSO4•7H2O
Filter sterilize into a sterile bottle or autoclave.
Stock 3:
- 4.0g/liter K2HPO4•3H2O
- or 3.05g/liter K2HPO4
Filter sterilize into a sterile bottle or autoclave.
Stock 5 (Microelements):
- 2.86g/liter H3BO3
- 1.81g/liter MnCl2•4H2O
- 0.222g/liter ZnSO4•7H2O
- 0.079g/liter CuSO4•5H2O
- 0.050g/liter COCl2•6H2O
- 0.391g/liter NaMoO4•2H2O
- or 0.018g/liter MoO4 (85%)
B) For basic BG11 medium combine the following stock solutions:
Stock Solution Per Liter of medium
- 10 mL Stock 1
- 10 mL Stock 2
- 10 mL Stock 3
- 0.02 g Na2CO3
- 1.0 mL Stock 5
- 1.5 g NaNO3
Combine stocks and adjust pH to 7.5 (use 1.0N HCl). Aliquot into flasks (50 ml/125 ml flask) with cotton stoppers on top and autoclave. After autoclaving and cooling the pH is about 7.1. For solid media add 1% noble agar. For BG-11, don't add NaNO3.
