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Team:Brown-Stanford/Lab/Protocols/BG11
From 2011.igem.org
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=== '''Stock solutions for BG-11''' === | === '''Stock solutions for BG-11''' === | ||
Stock 1: | Stock 1: | ||
| - | 0.1g/liter | + | 0.1g/liter Na<sub>2</sub>MG EDTA |
0.6g/liter Ferric ammonium citrate | 0.6g/liter Ferric ammonium citrate | ||
| - | Citric acid• | + | Citric acid•1H<sub>2</sub>O |
0.6g/liter | 0.6g/liter | ||
| - | + | CaCl<sub>2</sub>•2H<sub>2</sub>O | |
3.6g/liter | 3.6g/liter | ||
Filter sterilize into a sterile bottle or autoclave | Filter sterilize into a sterile bottle or autoclave | ||
Stock 2: | Stock 2: | ||
| - | + | MgSO<sub>4</sub>•7H<sub>2</sub>O | |
7.5g/liter | 7.5g/liter | ||
Filter sterilize into a sterile bottle or autoclave | Filter sterilize into a sterile bottle or autoclave | ||
Stock 3: | Stock 3: | ||
| - | + | K<sub>2HPO</sub>4•3H<sub>2</sub>O | |
4.0g/liter | 4.0g/liter | ||
| - | or | + | or K<sub>2</sub>HPO<sub>4</sub> |
3.05g/liter | 3.05g/liter | ||
Filter sterilize into a sterile bottle or autoclave | Filter sterilize into a sterile bottle or autoclave | ||
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H3BO3 | H3BO3 | ||
2.86g/liter | 2.86g/liter | ||
| - | + | MnCl<sub>2</sub>•4H<sub>2</sub>O | |
1.81g/liter | 1.81g/liter | ||
| - | + | ZnSO<sub>4</sub>•7H<sub>2</sub>O | |
0.222g/liter | 0.222g/liter | ||
| - | + | CuSO<sub>4</sub>•5H<sub>2</sub>O | |
0.079g/liter | 0.079g/liter | ||
| - | + | COCl<sub>2</sub>•6H<sub>2</sub>O | |
0.050g/liter | 0.050g/liter | ||
| - | + | NaMoO<sub>4</sub>•2H<sub>2</sub>O | |
0.391g/liter | 0.391g/liter | ||
| - | or | + | or MoO<sub>4</sub> (85%) |
0.018g/liter | 0.018g/liter | ||
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B) For basic BG11 medium combine the following stock solutions: | B) For basic BG11 medium combine the following stock solutions: | ||
Stock Solution Per Liter of medium | Stock Solution Per Liter of medium | ||
| - | Stock 1 | + | *10 mL Stock 1 |
| - | 10 | + | *10 mL Stock 2 |
| - | Stock 2 | + | *10 mL Stock 3 |
| - | 10 | + | *0.02 g Na<sub>2</sub>CO<sub>3</sub> |
| - | Stock 3 | + | *1.0 mL Stock 5 |
| - | + | *1.5 g NaNO<sub>3</sub> | |
| - | + | ||
| - | 0. | + | |
| - | + | ||
| - | 1.0 | + | |
| - | + | ||
| - | 1. | + | |
| - | Combine stocks and adjust pH to 7.5 (use 1.0N HCl). Aliquot into flasks (50 ml/125 ml flask) with cotton stoppers on top and autoclave. After autoclaving and cooling the pH is about 7.1. For solid media add 1% noble agar. For BG- | + | Combine stocks and adjust pH to 7.5 (use 1.0N HCl). Aliquot into flasks (50 ml/125 ml flask) with cotton stoppers on top and autoclave. After autoclaving and cooling the pH is about 7.1. For solid media add 1% noble agar. For BG-11, don't add NaNO<sub>3</sub>. |
{{:Team:Brown-Stanford/Templates/Foot}} | {{:Team:Brown-Stanford/Templates/Foot}} | ||
Revision as of 23:34, 11 June 2011
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REGObricks
Intro--------------------- ISRU--------------------- Biocement--------------------- S. pasteurii--------------------- Balloon
Flights--------------------- Transforming--------------------- Biobrick2 -
PowerCell
Intro--------------------- Cyanobacteria--------------------- Mars--------------------- Nutrients -
FRETcetera
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Lab
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Partners
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Outreach
SB5.0--------------------- NASA--------------------- Lunar Science--------------------- Maker Faire--------------------- BBC--------------------- CBricks--------------------- Ethics--------------------- Regional
Jamboree
BG-11
(Taken from [http://microbiology.ucdavis.edu/meeks/BG11medium.html|UC Davis])
Stock solutions for BG-11
Stock 1: 0.1g/liter Na2MG EDTA 0.6g/liter Ferric ammonium citrate Citric acid•1H2O 0.6g/liter CaCl2•2H2O 3.6g/liter Filter sterilize into a sterile bottle or autoclave Stock 2: MgSO4•7H2O 7.5g/liter Filter sterilize into a sterile bottle or autoclave Stock 3: K2HPO4•3H2O 4.0g/liter or K2HPO4 3.05g/liter Filter sterilize into a sterile bottle or autoclave Stock 5 (Microelements): H3BO3 2.86g/liter MnCl2•4H2O 1.81g/liter ZnSO4•7H2O 0.222g/liter CuSO4•5H2O 0.079g/liter COCl2•6H2O 0.050g/liter NaMoO4•2H2O 0.391g/liter or MoO4 (85%) 0.018g/liter
B) For basic BG11 medium combine the following stock solutions:
Stock Solution Per Liter of medium
- 10 mL Stock 1
- 10 mL Stock 2
- 10 mL Stock 3
- 0.02 g Na2CO3
- 1.0 mL Stock 5
- 1.5 g NaNO3
Combine stocks and adjust pH to 7.5 (use 1.0N HCl). Aliquot into flasks (50 ml/125 ml flask) with cotton stoppers on top and autoclave. After autoclaving and cooling the pH is about 7.1. For solid media add 1% noble agar. For BG-11, don't add NaNO3.
