Team:Amsterdam/9 August 2011

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09-08-2011 | Meeting 18

Synthesized genes

  • Ordering problems
    • Problem: it was divided in each line a different sequence/tube (1, 61, 181 etc.).


Labwork

Different constructs are being transformed to top10 and dh5alpha. A problem with miniprep, just with one construct The construct has been redone More results tomorrow

Wiki

  • Working on applications page


Extra

Antifreeze proteins, can be added to our project Yale has been contacted on their project for antifreeze proteins

  • Waiting for reply to send their bricks to us


Primers should be made for the new stuff from igem send last week

Multiple antifreeze proteins will be added to the project These will be also worked with on the lab These genes will also be used to increase the growth at low temperatures (might work on combination)


Modelling

Protein concentration ---- Fluorescence Miller units are these useful for a concentration conversion?

  • In Cell free chassis: GFP is a mutant but their characterisation of degradation time is strange

Labwork

Worked on constructs:

  • K206000+E0840 in bb tetra
  • K206000+E0840 in bb tetra
  • K206000+I732017 in bb tetra
  • K206001+I732017 in bb tetra
  • R0010+I732017+B0014 in bb chlora