Agenda: Examining Results of Establishment of the K346002 Hg Sensitive Promotor Round II
From 2011.igem.org
05/26/11 Agenda: Examining Results of Establishment of the K346002 Hg Sensitive Promotor Round II
(1) Results: NO GROWTH!
(2) Conclusion: Perhaps no transformation and bad AMP on 4-21-10 plate. However, could still be some transformants present.
(3) Q-tip swabbed up lawn and resuspended in AMP LB broth. Resuspension fluid placed in photospectrometer.
(4) Photospectrometer results for K346002:
Time A600 nm 0 hr. .315 1 hr. .148 2.5 hr. .133
(5) Results: absorbance is declining meaning cells are dying, thus no transformation has resulted. Will leave in incubator overnight to see if transformants grow out.
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