"

From 2011.igem.org

(1) Obtained a vial of 2mL of TOP-10 electro competent E. coli cells 3-16-11 (44-0003/793762)

(2) Added 50 microliters of BIO-RAD transformation solution 
   (Control number 31000008916 recd 1-17-11 GT)

(3) Added 2 microliters of DNA from Registry plate 2  well 5b

(4) Incubate on ice 5 minutes.

(5) Heat shocked 40 degrees C for 50 seconds

(6) Incubated 4 degrees C for 50 seconds

(7) Added 1mL LB 5-17-11 D.G.

(8) Plate 100 microliters and 25 microliters of transformed cells on LB-AMP (5-19-11 "who")

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