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Team:Groningen/project notebook/6 July 2011
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<br>* Colony PCR of 6 constructs! | <br>* Colony PCR of 6 constructs! | ||
<br>HybB promotor in pSB1CR | <br>HybB promotor in pSB1CR | ||
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<br>Final extension: 72° C for ten minutes | <br>Final extension: 72° C for ten minutes | ||
<br>Store at 4 ° C (infinite) | <br>Store at 4 ° C (infinite) | ||
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Latest revision as of 11:48, 20 September 2011
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Joyce
* Colony PCR of 6 constructs!
HybB promotor in pSB1CR
HybB promotor in RBS GFP ampicillin resistance vector
PcI promotor in RBS GFP ampicillin resistance vector
PlasI promotor in RBS GFP ampicillin resistance vector
LasI-LVA in ampicillin resistance with double terminator vector
cI-LVA in ampicillin resistance with double terminator vector
Colony PCR protocol:
Media: Taq PCR mastermix
Preheated Lid: 111° C
Denaturation: 95 ° C for ten minutes
Cycle 33×
Denaturation: 95° C for 30 seconds
Annealing: 60° C for 30 seconds
Extension: 72° C for 1 minute
Final extension: 72° C for ten minutes
Store at 4 ° C (infinite)
