Team:Groningen/project notebook/21 June 2011
From 2011.igem.org
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Joyce
Team meeting!
File:Meeting notes june 21.pdf
Digestion+ ligation PhybB product with taq polymerase
Purify the PCR product with the High Pure PCR Purification kit from Roche
Digestion mix:
18μl buffer
4.5μl EcoRI
4.5μl SpeI
49μl MQ water
Vector mix:
18μl buffer
4.5μl EcoRI
4.5μl XbaI
49μl MQ water
Ligation:
2μl T4 DNA ligase buffer
1μl T4 DNA ligase
7μl insert
3μl vector
7μl MQ water
Ligate 2 hours at room temperature
Ligate further overnight
Helping with sponsoring
Making researchplan