Joyce
PCR of HybB promotor, PcI, PlasI, LasR-LVA, cI LVA.
PCR protocol:
Denaturation: 94 °C 5 minutes
Cycle 30×
Denaturation: 94 °C 30 seconds
Annealing: 30 seconds 60 degrees
Extension: 2 minutes
Final extension: 7 minutes
Store infinite at 4 °C
Analyze on 1% agarose gel
Work on Human Practices
Jakub
Week 27:
- Cloning LasR variants into plasmids with reverse double terminator and LasB promoter - first try.
Christoph
- Cloning the last cI variants into the plasmid with DT+revPlasI, because some sequences seemd not alright.