Joyce
Repeat PCRs for PhybB, same conditions as before
Clean up and measure DNA concentration with the ND1000 nanodrop: 50ng/microliter on average per sample
Jaap
Tested our bistabilety analiser today and it worked like a charm. What it basically does is take a promotor description, a protein decay function and an rna halflife and predicts where (if any) the stable states of the sytem are goin to be. Afterwards you can use a full model to check how robust these points really are. To us that meant that we saw how extremly important it is to model the delay in transcription and translation.