Team:Fatih Turkey/Experiments

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<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a>
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<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li>
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<div class="bar-title-whole">
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  <div class="bar-title">
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      <h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Experiments</h2>
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      <h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Charactarization</h5>
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              <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/confirmation">Charactarization</a></li>
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              </li>
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 +
           
 +
                  <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments1">Exposure Experiment</a></li>
 +
                 
 +
               
 +
                  <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiment3C">Inhibition in Liquid Culture Assay</a></li>
 +
                  <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments4">Inhibition in Supernatant Assay</a></li>
 +
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments2">The Suicide Experiment of E.Coli</a></li>
 +
                  <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments5">Fenton Reagent Assay</a></li>
 +
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments6">Existence of Reflectin</a></li>
 +
             
 +
            </ol>
 +
        </div>
 +
        <div style="width: 654px; border: solid 3px #666; border-radius: 10px; padding-left: 80px; margin-left: 170px;">
 +
            <table width="606" height="203" border="0">
 +
  <tr>
 +
    <td width="600" valign="top"><p><strong>CHARACTERIZATION</strong><br />
 +
<p><center><img src="https://static.igem.org/mediawiki/2011/5/59/Chrac.png" width="500" /></center></p>
 +
      <p>Before starting assays; we needed to be sure that our genes had been cloned  correctly. In order to use our parts in our experiments, we confirmed our parts  via electrophoresis. Following parts are fully confirmed:</p>
 +
      <table border="1" cellspacing="0" cellpadding="0">
 +
        <tr>
 +
          <td width="307" height="35" valign="top"><strong>Subparts</strong> </td>
 +
          <td width="307" valign="top"><p><strong>Parts</strong></p></td>
 +
        </tr>
 +
        <tr>
 +
          <td width="307" valign="top"><p>BBa_K541501</p></td>
 +
          <td width="307" valign="top"><p>BBa_K541515</p></td>
 +
        </tr>
 +
        <tr>
 +
          <td width="307" valign="top"><p>BBa_K541502</p></td>
 +
          <td width="307" valign="top"><p>BBa_K541545</p></td>
 +
        </tr>
 +
        <tr>
 +
          <td width="307" valign="top"><p>BBa_K541503</p></td>
 +
          <td width="307" valign="top"><p>BBa_K541596</p></td>
 +
        </tr>
 +
        <tr>
 +
          <td width="307" valign="top"><p>BBa_K541504</p></td>
 +
          <td width="307" valign="top"><p>BBa_K541915</p></td>
 +
        </tr>
 +
        <tr>
 +
          <td width="307" valign="top"><p>BBa_K541505</p></td>
 +
          <td width="307" valign="top"><p>BBa_K541800</p></td>
 +
        </tr>
 +
        <tr>
 +
          <td width="307" valign="top"><p>BBa_K541506</p></td>
 +
          <td width="307" valign="top"><p>BBa_K541516</p></td>
 +
        </tr>
 +
        <tr>
 +
          <td width="307" valign="top"><p>&nbsp;</p></td>
 +
          <td width="307" valign="top"><p>BBa_K541526</p></td>
 +
        </tr>
 +
        <tr>
 +
          <td width="307" valign="top"><p>&nbsp;</p></td>
 +
          <td width="307" valign="top"><p>BBa_K541546</p></td>
 +
        </tr>
 +
      </table>
 +
      <p>After electrophoresis results; we furthermore performed sequencing and  imaging tests to characterize our BioBricks. For BBa_541505 and BBa_541506; we  added a GFP sequence and transformated into E.coli. This has characterized our  main proteins. cDNA sequencing for these parts was also done successfully.<br />
 +
        In addition, BBa_541800 is transformated into B.subtilis and successfully  imaged red that shows RFP region and therefore plasmid backbone works properly.<br />
 +
    We characterized BBa_K541545, BBa_K541515 and BBa_K541915 via sequencing.</p></td>
 +
  </tr>
 +
</table>
 +
<p><center><img src="https://static.igem.org/mediawiki/2011/b/bc/Jelgoruntusu.jpg" width="500" /></center></p>
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Latest revision as of 01:36, 29 October 2011

deneme baslik

Experiments

Charactarization

CHARACTERIZATION

Before starting assays; we needed to be sure that our genes had been cloned correctly. In order to use our parts in our experiments, we confirmed our parts via electrophoresis. Following parts are fully confirmed:

Subparts

Parts

BBa_K541501

BBa_K541515

BBa_K541502

BBa_K541545

BBa_K541503

BBa_K541596

BBa_K541504

BBa_K541915

BBa_K541505

BBa_K541800

BBa_K541506

BBa_K541516

 

BBa_K541526

 

BBa_K541546

After electrophoresis results; we furthermore performed sequencing and imaging tests to characterize our BioBricks. For BBa_541505 and BBa_541506; we added a GFP sequence and transformated into E.coli. This has characterized our main proteins. cDNA sequencing for these parts was also done successfully.
In addition, BBa_541800 is transformated into B.subtilis and successfully imaged red that shows RFP region and therefore plasmid backbone works properly.
We characterized BBa_K541545, BBa_K541515 and BBa_K541915 via sequencing.



2011 © Fatih Medical School

Retrieved from "http://2011.igem.org/Team:Fatih_Turkey/Experiments"