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Team:British Columbia/Notebook/Week 2
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==June 13 2011== | ==June 13 2011== | ||
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Daisy is working with the synthase 3-carene; 3-carene has three restriction sites that need to be modified (EcoRI, XbaI, PstI). She worked over the weekend, and has one more site to modify. | Daisy is working with the synthase 3-carene; 3-carene has three restriction sites that need to be modified (EcoRI, XbaI, PstI). She worked over the weekend, and has one more site to modify. | ||
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==June 18 2011== | ==June 18 2011== | ||
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Daisy did a multiple site-directed mutagenesis using three sets of forward and reverse primers which contain the desired changes. | Daisy did a multiple site-directed mutagenesis using three sets of forward and reverse primers which contain the desired changes. | ||
Revision as of 15:40, 3 August 2011
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June 13 2011
3-Carene Daisy is working with the synthase 3-carene; 3-carene has three restriction sites that need to be modified (EcoRI, XbaI, PstI). She worked over the weekend, and has one more site to modify.
We learned that the truncated version of the synthase will increase the efficiency of the enzyme.
June 15 2011
Canucks lost to the Bruins. It was a sad day for all...
June 18 2011
3-Carene Daisy did a multiple site-directed mutagenesis using three sets of forward and reverse primers which contain the desired changes.
