Team:British Columbia/Notebook/Week 2

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<div id="bod"><b> Week 2: June 12-18</b> <a name="w2"></a>
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==June 13 2011==
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==Lab Meeting - June 13==
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Overall, we had a productive meeting; we received updates on wetlab and modeling.
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Daisy is working with the synthase 3-carene; 3-carene has three restriction sites that need to be modified (EcoRI, XbaI, PstI). She worked over the weekend, and has one more site to modify.
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Daisy has started work on the 3-carene synthase, and Gurpal is working on getting the erg20-2 mutant yeast plasmid delivered from France.
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We learned that the truncated version of the synthase will increase the efficiency of the enzyme.
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The modeling team met with different UBC professors to discuss the model and to ask for advice.
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==June 15 2011==
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==3-Carene==
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Canucks lost to the Bruins. It was a sad day for all...
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[[File:Daisykissprare.jpg | thumb | left | 150px]]
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==June 18 2011==
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Daisy is working with the 3-carene monoterpene synthase that contains three restriction sites that need to be removed: EcoRI, XbaI, PstI. She worked hard, SDMing away over the weekend and has now only one more site to modify. We learnt from Chris Keeling that the truncated version of the synthase will increase the efficiency of the enzyme.
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Daisy did a multiple site-directed mutagenesis using three sets of forward and reverse primers which contain the desired changes.
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<br><br><br><br><br><br><br><br><br><br>
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==In other news...==
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<html><img src="http://upload.wikimedia.org/wikipedia/commons/2/2e/Vancouver_Riot_2011.jpg" height=200px border=1px></html>
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The Vancouver Canucks lost to the Boston Bruins. It was a sad day for all...
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Maybe next year, the cup will be ours. Just maybe.
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<a href="https://2011.igem.org/Team:British_Columbia/Notebook"><center><b>Back to the Notebook</b></center></a>

Latest revision as of 23:07, 16 October 2011

Team: British Columbia - 2011.igem.org

Week 2: June 12-18

Lab Meeting - June 13

Overall, we had a productive meeting; we received updates on wetlab and modeling.

Daisy has started work on the 3-carene synthase, and Gurpal is working on getting the erg20-2 mutant yeast plasmid delivered from France.

The modeling team met with different UBC professors to discuss the model and to ask for advice.

3-Carene

Daisykissprare.jpg

Daisy is working with the 3-carene monoterpene synthase that contains three restriction sites that need to be removed: EcoRI, XbaI, PstI. She worked hard, SDMing away over the weekend and has now only one more site to modify. We learnt from Chris Keeling that the truncated version of the synthase will increase the efficiency of the enzyme.











In other news...

The Vancouver Canucks lost to the Boston Bruins. It was a sad day for all... Maybe next year, the cup will be ours. Just maybe.


Back to the Notebook

Retrieved from "http://2011.igem.org/Team:British_Columbia/Notebook/Week_2"