Monoterpene Synthase Structural Modeling
Objective: To identify mutations that may enhance the efficiency of terpene synthesis, we constructed three-dimensional models of the terpene synthase proteins.
Model Creators: Joe Ho, Samuel Wu
Model Advisors: Shing Hei Zhan, Alina Chan
Three-dimensional structure of the 3 terpene synthases
Superimposition of the 3D structures of alpha-pinene synthase (blue), beta-pinene synthase (red), and limonene synthase (green). We used MODELLER (1) to automate homology-based 3D structure prediction. We identified an experimentally determined 3D structure of a taxadiene synthase from Pacific Yew (PDB ID: 3P5R) as an appropriate template.
Reactive site of the three synthases
Future Directions
References
1. N. Eswar, M. A. Marti-Renom, B. Webb, M. S. Madhusudhan, D. Eramian, M. Shen, U. Pieper, A. Sali. Comparative Protein Structure Modeling With MODELLER. Current Protocols in Bioinformatics, John Wiley & Sons, Inc., Supplement 15, 5.6.1-5.6.30, 2006.
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