Team:British Columbia/Model3

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Revision as of 02:39, 29 October 2011

Team: British Columbia - 2011.igem.org

Monoterpene Synthase Structural Modeling

Objective: To identify amino acid residues that are terpene synthesis, we constructed three-dimensional models of the terpene synthase proteins.

Model Creators: Joe Ho, Samuel Wu

Model Advisors: Shing Hei Zhan, Alina Chan

Three-dimensional structure of the 3 terpene synthases

Superimposition of the 3D structures of alpha-pinene synthase (blue), beta-pinene synthase (red), and limonene synthase (green). We used MODELLER (1) to automate homology-based 3D structure prediction. We identified an experimentally determined 3D structure of a taxadiene synthase from Pacific Yew (PDB ID: 3P5R) as an appropriate template.

Reactive site of the three synthases

Magnesium ions are cofactors located at the reactive site.

Future Directions

References

1. N. Eswar, M. A. Marti-Renom, B. Webb, M. S. Madhusudhan, D. Eramian, M. Shen, U. Pieper, A. Sali. Comparative Protein Structure Modeling With MODELLER. Current Protocols in Bioinformatics, John Wiley & Sons, Inc., Supplement 15, 5.6.1-5.6.30, 2006.

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 #bod {width:935px; float:left; background-color: white; margin-left: 15px;  margin-top:10px;}
 </style><div id="bod"><center><h3>Monoterpene Synthase Structural Modeling</h3></center></html>
-<b>Objective:</b> To identify mutations that may enhance the efficiency of terpene synthesis, we constructed three-dimensional models of the terpene synthase proteins.<p></p>
+<b>Objective:</b> To identify amino acid residues that are terpene synthesis, we constructed three-dimensional models of the terpene synthase proteins.<p></p>
 <b>Model Creators:</b> Joe Ho, Samuel Wu <p></p>
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 <b>Reactive site of the three synthases</b><p></p>
 [[File:UBCiGEM model3 reactive site.jpg]]
+Magnesium ions are cofactors located at the reactive site.
 <html><h3>Future Directions</h3></html><p></p>