Team:Amsterdam/Notebook/Protocols/Making electrocompetent Cells

From 2011.igem.org

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=Preparation of electrocompetent cells=
=Preparation of electrocompetent cells=
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==Overview==
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We use a protocol commonly used in our host lab provided by [https://2011.igem.org/Team:Amsterdam/Team/Advisors Diewertje Piebes].
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===Materials===
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===Procedure===
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*Prepare TOP10 preculture by inoculating 3ml of LB medium with a -80d°C TOP10 glycerol stock into and shake overnight at 37°C
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Revision as of 08:33, 21 September 2011

Contents

Preparation of electrocompetent cells

Overview

We use a protocol commonly used in our host lab provided by Diewertje Piebes.

Materials

Procedure

  • Prepare TOP10 preculture by inoculating 3ml of LB medium with a -80d°C TOP10 glycerol stock into and shake overnight at 37°C






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