Team:UNIST Korea/project/future work

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<font size=6><font color=cc0099><center>Future work & Application</center></font></font><br/><br/>
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Future work & Application </b></font><br/>
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<p align="justify">In the future our first effort will be to optimize the lysis module such that the Dpn enzymes are efficiently exploited by the synthetic microbes to eradicate its genetic material in a non-native environment.  
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As a future work of UNIST 2011 iGEM team, there will be additional circuits in our whole construct which will contain productive options for other applications. <br/> <br/>
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On top of this, UNIST team will also introduce more controlled system into <i>E.coli</i> so that it can be more safe to the environment and easy to be handled in the laboratory. We would like to introduce the following components into our <i>Chop. coli</i> in order to achieve our goal;<br/><br/>
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On the top of this, UNIST team is going to introduce more controlled system into our genetically modified <i>E.coli</i> so that it can be more safety and easy to be handled in the laboratory. Thus, one more light receptor, <font size=4.5><b>PhyB-PIF3</b></font> from Arabidopsis, which will play a key role for the control of gene expression will take a part in our future construct, which means even if light is present for a short time our system would not be willing to work fine if the light kept being present. <br/>
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<center><b><font color=cc0066><font size="5">Second light receptor&nbsp;&nbsp;&&nbsp;&nbsp;Quorum Sensing System</font></font></b></center><br/></p>
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Secondly, our team also will give a circuit containing <b><font size=4.5>quorum sensing system</b></font> to engineered organism. However, the quorum sensing system which we are going to introduce to <i>E.coli</i> is somewhat different from that of original one that <i>E.coli</i> has already. In case of original quorum sensing of <i>E.coli</i> in the nature, they produce the molecules, AI2s which are referred to auto inducer molecules which will directly go through the membrane, make the complexes with other binding proteins in cytoplasm, and eventually will affect the certain gene on the chromosomal DNA so that it can induce the expression as for positive feedback loop.
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Unlike the original quorum sensing system, UNIST team is going to make that engineered <i>E.coli</i> can only recognize the signals from different quorum sensing molecules, AHLs which come especially from different species.
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<p align="justify">Two light signals before lysis would help in providing a more tight control and ensure that the device would still work even when one of them is mutated.Our team  will also introduce a fourth sensing module to the <i>Chop. coli</i> using the quorum sensing system. In case of fermentor <i>E. coli</i> will experience its native original quorum sensing molecule, AI2
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Therefore, this system will also be the benefit to the effective control way of gene expressions. </br>
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UNIST team is going to make that engineer <i>E.coli</i> to recognize different quorum sensing molecules such as AHL to differentiate between the fermentor and the real world.
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This system will also help achieve an effective and tight control of lysis device. </br>
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Thirdly, We have been trying to concentrate on more accurate control of gene expressions and effective decrease of leaky levels in our circuits by designing the <b><font size=4.5>temperature-dependent mRNA secondary structure</b></font>, but since it might not be working well completely for now probably this is expected to be accomplished with more clear experimental data, which will play a key role in the future construct. </br>  
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Fourthly, We also have been focusing on making good lysis module introduced in our <i>E.coli</i> using either <b><font size=4.5>Holin system</b></font> or <font size=4.5><b>Dpn system</b></font>. Unfortunately, the result was not that clear as much as we expected. Thus, for the greater progress, UNIST iGEM team is determined to work on the experiment much about lysis system in the future. </br>  
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<center><b><font color=cc0066><font size="5">With this approach it is also possible to create artificial cell<br/><br/> membrane for the transplantation of synthetic genome. </font></font></b></center><br/></p>
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<center> <img src="https://static.igem.org/mediawiki/2011/d/d5/SKL30.png" width="500" height="500"/>
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Lastly, <font size=4.5><b>additional productive circuit</b></font> may join to the whole construct in the long future in order that <i>E.coli</i> can produce something genuinely valuable to the other things for applications because they may not be much worthy if they have nothing to produce anything although lots of controlled systems exist in the whole construct.
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For instance, as the additional productive systems for real applications, there might be systems containing the genes which are going to be much beneficial to the environment, or human health & well being, etc.
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<center><b>Figure explaining the future appearance of <i>Chop. coli</i></b></center>
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Latest revision as of 02:36, 6 October 2011


Future work & Application


In the future our first effort will be to optimize the lysis module such that the Dpn enzymes are efficiently exploited by the synthetic microbes to eradicate its genetic material in a non-native environment. On top of this, UNIST team will also introduce more controlled system into E.coli so that it can be more safe to the environment and easy to be handled in the laboratory. We would like to introduce the following components into our Chop. coli in order to achieve our goal;

Second light receptor  &  Quorum Sensing System

Two light signals before lysis would help in providing a more tight control and ensure that the device would still work even when one of them is mutated.Our team will also introduce a fourth sensing module to the Chop. coli using the quorum sensing system. In case of fermentor E. coli will experience its native original quorum sensing molecule, AI2 UNIST team is going to make that engineer E.coli to recognize different quorum sensing molecules such as AHL to differentiate between the fermentor and the real world. This system will also help achieve an effective and tight control of lysis device.


With this approach it is also possible to create artificial cell

membrane for the transplantation of synthetic genome.


Figure explaining the future appearance of Chop. coli

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