Team:UNIST Korea

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<br/>
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<font face=calibri><font size="9"><font color="cc0099"><center>CHOp-Coli-LATE</p></font></font>
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<font size="6"><font color=cc0066><center>Synthetic Microbes</p></font></font><br/>
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<p align="justify"><font size=4 color=maroon>Synthetic Microbes are gaining increased importance in 21 st century. Synthetic Microbes are more promising hosts for fuel production, bioremediation and many other potential processes. There is an increased dependence on the synthetic microbes even for certain basic needs such as medicine and fuels. However, synthetic microbes do not experience a warm welcome from the general public because they are believed to endanger the environment.Synthetic Microbes: <br/><br/></P>
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<font size="5"><font color="Red">C</font></font>an escape from its restricted area and can
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<br/>  <font size="5"><font color="Red">H</font></font>orizontally tranfer its genetic materials to pathogens leading to super bugs that can go <br/>
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<font size="5"><font color="Red">O</font></font>ut of control of human and<br/>
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<font size="5"><font color="Red">C</font></font>ause epidemics; can <br/>
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<font size="5"><font color="Red">L</font></font>ethal biological weapon hence synthetic microbes are being <br/>
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<font size="5"><font color="Red">A</font></font>bjured by the world as they<br/>
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<font size="5"><font color="Red">T</font></font>hreaten the existing biodiversity and are believed to be <br/>
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<font size="5"><font color="Red">E</font></font>nvironmentally damaging                        <font size="5"><font color="Red">ELYZIANS</font></font> <br/><br/></p><br/>
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<p align="justify">All advances in synthetic biology is because of the tiny double helical molecule called  <font size="5"><font color="Red">DNA</font></font>. All threats of synthetic biology is again because of these tiny molecules called <font size="5"><font color="Red">DNA</font></font>. Thus, DNA forms two sides of the same coin. <br /></font><br/><br/></font></p>
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<font size="6"><font color=cc0066> Overview of CHOCOLATE</font></font><br/><br/>
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<p style="float:right"><img src="https://static.igem.org/mediawiki/2011/a/a3/Skl32.jpg" height="290" width="250"></p><p align="justify">CHOCOLATE project aims at eradicating the negative aspects of synthetic biology by exploiting the defence mechanism used by natural microbes to differentiate its genome from that of the foreign DNA. Natural microbes utilize a group of restriction enzymes like dam, dcm and dpn to diferentiate its DNA from that of the foreign DNA. We simply modified the host defence machanism such that the synthetic microbe would identify its genome as its native when it stays in its desired environment and the host defence will be switched OFF. However, when the synthetic microbe senses that it is in a non-native environment it would turn ON its host defence to destroy its own DNA and kill itself. Thus, this system would not only favor cell death but also ensure that all the genetic materials are destroyed and guarantee that there would be no horizontal gene transfer. </font></font></img>
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<a href="https://2011.igem.org/Team:KAIST-Korea/jihan"><img id="tag1" src="https://static.igem.org/mediawiki/2011/8/8f/Kaist2011_text_home.png" />
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<h2> <span class="mw-headline" id="Project_Description">Project Description</span></h2>
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<p><br />
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E. Casso is an E. coli system engineered into art. This system consists of two types of genetically modified E. coli: the random signal generating E. coli (the “Brush E. coli”) and the color generating E. coli (the “Paint E. coli”).
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</p><p><br />
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The Brush E. coli possesses genes (lasI, luxI, rhlI, and cinI) that produce quorum in its chromosomal DNA. Each Brush E. coli will secrete quorum molecules randomly associated with one of four colors among red, cyan, yellow, and green by a modified Cre-loxP mechanism.
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</p><p><br />
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The Paint E.coli possesses plasmid vectors that express one of four colors of fluorescent proteins in response to the type of quorum it receives from the Brush E.coli. The quorum sensing complex completed in the Brush will bind to the ribosome binding site (RBS) of the corresponding fluorescent gene.
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</p><p><br />
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Using this system, we can create art on an LP plate with the cells' “random” contribution.
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Latest revision as of 14:34, 5 October 2011


CHOp-Coli-LATE

Synthetic Microbes


Synthetic Microbes are gaining increased importance in 21 st century. Synthetic Microbes are more promising hosts for fuel production, bioremediation and many other potential processes. There is an increased dependence on the synthetic microbes even for certain basic needs such as medicine and fuels. However, synthetic microbes do not experience a warm welcome from the general public because they are believed to endanger the environment.Synthetic Microbes:

Can escape from its restricted area and can
Horizontally tranfer its genetic materials to pathogens leading to super bugs that can go
Out of control of human and
Cause epidemics; can
Obdurately occupy the universe and can be a
Lethal biological weapon hence synthetic microbes are being
Abjured by the world as they
Threaten the existing biodiversity and are believed to be
Environmentally damaging ELYZIANS


DNA is two sides of the same coin

All advances in synthetic biology is because of the tiny double helical molecule called DNA. All threats of synthetic biology is again because of these tiny molecules called DNA. Thus, DNA forms two sides of the same coin.


Overview of CHOCOLATE

CHOCOLATE project aims at eradicating the negative aspects of synthetic biology by exploiting the defence mechanism used by natural microbes to differentiate its genome from that of the foreign DNA. Natural microbes utilize a group of restriction enzymes like dam, dcm and dpn to diferentiate its DNA from that of the foreign DNA. We simply modified the host defence machanism such that the synthetic microbe would identify its genome as its native when it stays in its desired environment and the host defence will be switched OFF. However, when the synthetic microbe senses that it is in a non-native environment it would turn ON its host defence to destroy its own DNA and kill itself. Thus, this system would not only favor cell death but also ensure that all the genetic materials are destroyed and guarantee that there would be no horizontal gene transfer.

Retrieved from "http://2011.igem.org/Team:UNIST_Korea"