Team:Fatih Turkey/Experiments4

From 2011.igem.org

(Difference between revisions)
Line 568: Line 568:
         <div style="float: left; width: 220px; border-radius: 10px; position: absolute; top: 50px; left: 0; background: #EEE; border: solid 3px #666;">
         <div style="float: left; width: 220px; border-radius: 10px; position: absolute; top: 50px; left: 0; background: #EEE; border: solid 3px #666;">
             <ol>
             <ol>
-
               <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/confirmation">Confirmation</a></li>
+
               <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/confirmation">Charactarization</a></li>
                
                
               </li>
               </li>
Line 575: Line 575:
                   <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments1">Exposure Experiment</a></li>
                   <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments1">Exposure Experiment</a></li>
                    
                    
-
                  <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments2">The Suicide Experiment of E.Coli</a></li>
 
                   <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiment3C">Inhibition in Liquid Culture Assay</a></li>
                   <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiment3C">Inhibition in Liquid Culture Assay</a></li>
                   <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments4">Inhibition in Supernatant Assay</a></li>
                   <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments4">Inhibition in Supernatant Assay</a></li>
 +
 +
                  <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments2">The Suicide Experiment of E.Coli</a></li>
                   <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments5">Fenton Reagent Assay</a></li>
                   <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments5">Fenton Reagent Assay</a></li>
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments6">Existence of Reflectin</a></li>
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments6">Existence of Reflectin</a></li>
Line 584: Line 585:
         </div>
         </div>
         <div style="width: 654px; border: solid 3px #666; border-radius: 10px; padding-left: 80px; margin-left: 170px;">
         <div style="width: 654px; border: solid 3px #666; border-radius: 10px; padding-left: 80px; margin-left: 170px;">
-
            <p>asdada</p><p>asdada</p><p>asdada</p><p>asdada</p><p>asdada</p><p>asdada</p><p>asdada</p><p>asdada</p>
+
         
-
<br><br><br><br><br><br><br><br><br><br>
+
<table width="606" height="227" border="0">
 +
  <tr>
 +
    <td width="600" height="223" valign="top"><p><strong>Inhibition  in Supernatant Assay</strong><br />
 +
      In this experiment, we wanted to investigate the effects of supernatant  includes LALF protein to E.coli. Therefore, we put 8 ul LB broth into falcons.  We added them E.coli with RFP and incubated for 12 hours in 37⁰C .We put pure E.coli into only one falcon for  control. We added 1ul, 10ul and 100ul supernatant with LALF and without LALF.<br />
 +
    We diluated them 7 times after 2 – 4 – 8 – 12 hours. Then, we spread 10 ul  into plates which has half volume of spread amount chloramphenicol. We  centrifuged the liquid cultures which incubated for 12 hours at 37⁰C at 7000 rpm for 5 minutes because of preparing  supernatant with LALF and without LALF. All supernatants were passed through  the 0,2 filter in order to decontaminate from bacteria and their disposals.</p></td>
 +
  </tr>
 +
</table>
 +
 
         </div>
         </div>
     </div>
     </div>

Revision as of 15:53, 28 October 2011

deneme baslik

Experiments

Inhibition in Supernatant Assay

Inhibition in Supernatant Assay
In this experiment, we wanted to investigate the effects of supernatant includes LALF protein to E.coli. Therefore, we put 8 ul LB broth into falcons. We added them E.coli with RFP and incubated for 12 hours in 37⁰C .We put pure E.coli into only one falcon for control. We added 1ul, 10ul and 100ul supernatant with LALF and without LALF.
We diluated them 7 times after 2 – 4 – 8 – 12 hours. Then, we spread 10 ul into plates which has half volume of spread amount chloramphenicol. We centrifuged the liquid cultures which incubated for 12 hours at 37⁰C at 7000 rpm for 5 minutes because of preparing supernatant with LALF and without LALF. All supernatants were passed through the 0,2 filter in order to decontaminate from bacteria and their disposals.
2011 © Fatih Medical School

Retrieved from "http://2011.igem.org/Team:Fatih_Turkey/Experiments4"