Week 2: Monday May 2 - Friday May 6

Monday

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Monoterpene Synthase Project

PsTPS-Pin:

    Objective: PCR with designed primers for Site Directed Mutagenesis following the Stratagene Quikchange Protocol. 
    Procedures: Following the protocol from Stratagene Quikchange Protocol
    Results: No PCR products were amplified after gel check with gel electrophoresis. Primers are visible on the gel. 
             Possible degradation of the Pfu polymerase, which explains why the PCR has not worked