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Team:British Columbia/Week2
From 2011.igem.org
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Monoterpene Synthase Project''' | Monoterpene Synthase Project''' | ||
Revision as of 04:28, 29 June 2011
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Week 2: Monday May 2 - Friday May 6
Monday
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Monoterpene Synthase Project
PsTPS-Pin:
Objective: PCR with designed primers for Site Directed Mutagenesis following the Stratagene Quikchange Protocol.
Procedures: Following the protocol from Stratagene Quikchange Protocol
Results: No PCR products were amplified after gel check with gel electrophoresis. Primers are visible on the gel.
Possible degradation of the Pfu polymerase, which explains why the PCR has not worked
