Team:British Columbia/Objectives

From 2011.igem.org

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<h3><center>Wet Laboratory</center></h3></html>
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==Wet Lab==
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[[File:ubcigem2011comic4.jpg | center ]]
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'''Production of Monoterpenes in Yeast'''
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From an industrial perspective, this yeast will be cultured and harvested for monoterpenes, which will be analysed by gas chromatography mass spectrometry (GC-MS). Yeast is a highly utilized organism in industry (e.g. breweries and also the recently established Amyris biotech company that uses yeast to produce anti-Malarial therapeutics amongst other valuable compounds). We envision that monoterpene-producing yeast will not only be industrially profitable, but also help to alleviate the unsustainable consumption of nature's resources in the quest for biofuels, pharmaceuticals, flavours/fragrances etc.
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From an environmental perspective, this yeast will be co-cultured with the bluestain fungus to determine if monoterpene-producing yeast is able to inhibit the growth of the fungus. This yeast is also a biological system that can be screened for potential targets against the fungus. For instance, since the yeast is actively producing monoterpenes, it is possible to look for yeast that have particular mutations that cause increased intolerance against monoterpenes. These mutations will reveal potential strategies against the fungus.
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'''Monoterpene Synthases'''
'''Monoterpene Synthases'''
Our goal is to express synthases that produce monoterpenes (specifically limonene, pinene, carene and cineole) in yeast. Genes encoding these synthases will be inserted into yeast gateway vectors as well as the biobrick plasmid PSB1C3.
Our goal is to express synthases that produce monoterpenes (specifically limonene, pinene, carene and cineole) in yeast. Genes encoding these synthases will be inserted into yeast gateway vectors as well as the biobrick plasmid PSB1C3.
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'''Metabolic Genes'''
'''Metabolic Genes'''
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Our goal is to express metabolic enzymes (specifically, erg20-2, Idi1 and Hmg2) that optimize the mevalonate pathway in yeast for monoterpene production.
Our goal is to express metabolic enzymes (specifically, erg20-2, Idi1 and Hmg2) that optimize the mevalonate pathway in yeast for monoterpene production.
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<html><a href="https://2011.igem.org/Team:British_Columbia/Characterized">Characterization details here!</a></html>
 
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'''Production of Monoterpenes in Yeast'''
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==Modeling==
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From an industrial perspective, this yeast will be cultured and harvested for monoterpenes, which will be analysed by gas chromatography mass spectrometry (GC-MS). Yeast is a highly utilized organism in industry (e.g. breweries and also the recently established Amyris biotech company that uses yeast to produce anti-Malarial therapeutics amongst other valuable compounds). We envision that monoterpene-producing yeast will not only be industrially profitable, but also help to alleviate the unsustainable consumption of nature's resources in the quest for biofuels, pharmaceuticals, flavours/fragrances etc.
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'''Monoterpene Production in Yeast'''
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From an environmental perspective, this yeast will be co-cultured with the bluestain fungus to determine if monoterpene-producing yeast is able to inhibit the growth of the fungus. This yeast is also a biological system that can be screened for potential targets against the fungus. For instance, since the yeast is actively producing monoterpenes, it is possible to look for yeast that have particular mutations that cause increased intolerance against monoterpenes. These mutations will reveal potential strategies against the fungus.
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Our goal is to develop a model to assay the secretion and production of monoterpenes in yeast.  
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<html><b><a href="https://2011.igem.org/Team:British_Columbia/Data">Characterization details here!</a></b></html>
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'''Mountain Pine Beetle Epidemic'''
 
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Our goal is to construct a mathematical model that can predict the dynamics of the mountain pine beetle populations in B.C. under the influence of our synthetic product. This model can be coupled with the strategic planning for future conservation initiatives.
 
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<html><a href="https://2011.igem.org/Team:British_Columbia/Modeling">Modeling details here!</a></html>
 
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<html><h3><center>Computational Modeling</center></h3></html>
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==Human Practices==
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  <area shape="circle" coords="490,290,80" href="https://2011.igem.org/Team:British_Columbia/Model1" />
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  <area shape="circle" coords="750,290,70" href="https://2011.igem.org/Team:British_Columbia/Model3" />
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<Center><div id="header"><img style="margin-bottom:0px; margin-top:3px; margin-left: -5px; padding:0;" src="https://static.igem.org/mediawiki/2011/e/e7/Ubcigemmodelmini.jpg" usemap="#modelheadermap" alt="UBC iGEM 2011 human practices header"> </div></center>
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</html>
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'''1. Build bridges between individuals, communities and countries.'''
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'''Mountain Pine Beetle Epidemic'''
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* The focus is not ourselves, but bridging the gap between researchers in synthetic biology and people outside the field.
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A mathematical model that can predict the dynamics of the mountain pine beetle populations in B.C. under the influence of our synthetic product. This model can be coupled with the strategic planning for future conservation initiatives.  
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'''2. Actively seek out mutually transforming relationships.'''
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'''Monoterpene Production in Yeast'''
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* We do not want a one-way relationship - we want symbiosis!
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A model that can assay the production of monoterpenes in our genetically engineered yeast cells given that they contain the metabolic genes IDI1, K6R-hmg2, erg20-2 and a monoterpene synthase.
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* We aim to learn as much as we teach.
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'''Synthase Structure'''
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'''3. Use multimedia tools to reach the community in an engaging way.'''
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A model that can determine monoterpene synthase product specificity and enzymatic activity based on amino acid sequence.
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* We want to share our enthusiasm for our iGEM project and inspire people to see how this technology can serve their unique passions
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<html><b><a href="https://2011.igem.org/Team:British_Columbia/Modeling">Modeling details here!</b></a></html>
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* Outreach at <html><a href="http://www.scienceworld.ca/">Science World</a></html>
 
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* Deploy iGEM outreach resources (http://openwetware.org/wiki/IGEM_Outreach)
 
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'''4. Create a universal accessible iGEM language'''
 
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* Compile an iGEM dictionary for synthetic biology and iGEM specific terms
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<html><h3><center>Human Practices</center></h3></html>
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  <area shape="circle" coords="100,100,60" href="https://2011.igem.org/Team:British_Columbia/Mentorship" />
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  <area shape="circle" coords="300,450,60" href="https://2011.igem.org/Team:British_Columbia/Wild" />
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  <area shape="circle" coords="500,100,60" href="https://2011.igem.org/Team:British_Columbia/Perceptions" />
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<div id="wrap">
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<Center><div id="header"><img style="margin-bottom:0px; margin-top:3px; margin-left: -5px; padding:0;" src="https://static.igem.org/mediawiki/2011/7/7a/Ubcigemhp.jpg" width=600px usemap="#hpheadermap" alt="UBC iGEM 2011 human practices header"> </div></center></html>
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* Collaborate with other iGEM teams
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'''Mentorship'''
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'''5. Invest in the FUTURE and pursue sustainable projects'''
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#Outreach at '''<html><a href="http://www.scienceworld.ca/">Science World</a></html>''': Start a high school iGEM team and mentor students participating in the '''<html><a href="http://www.scienceworld.ca/futurescienceleaders">Future Leaders in Science</a></html>''' program
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#Initiate collaboration with other iGEM teams to compile an <html><a href="http://openwetware.org/wiki/IGEM_Outreach:iGEM_Dictionary">iGEM dictionary</a></html> for synthetic biology and iGEM specific terms
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* Live in a way that incorporates those outside the lab into our comfort zone and personal passions for research, iGEM and synthetic biology.
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'''Public Perceptions'''
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* Start a high school iGEM team and mentor students participating in the <html><a href="http://www.scienceworld.ca/futurescienceleaders">Future Leaders in Science</a></html> program at Science World
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#Inform hundreds in the UBC student community about our project and iGEM at '''[http://www.students.ubc.ca/newtoubc/orientations.cfm?page=imagine Imagine Day Orientation]'''
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#Tracking changes in '''[https://2011.igem.org/Team:British_Columbia/Perceptions public perception]''' by asking for and collating definitions of synthetic biology each year at UBC
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* Inform UBC students about our project and iGEM club at Clubs Day and Imagine Week
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'''Synthetic Biology in the Wild'''
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* Recruit new team members for next year!
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#Conduct '''[https://2011.igem.org/Team:British_Columbia/Wild interviews]''' with experts in relevant fields to review the implications of introducing synthetic organisms into the environment
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<html><a href="https://2011.igem.org/Team:British_Columbia/HP">Multimedia and outreach details here!</a></html>
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<html><b><a href="https://2011.igem.org/Team:British_Columbia/HP">Multimedia and outreach details here!</b></a></html>

Latest revision as of 21:31, 21 October 2011

Team: British Columbia - 2011.igem.org

Wet Laboratory

Ubcigem2011comic4.jpg

Monoterpene Synthases

Our goal is to express synthases that produce monoterpenes (specifically limonene, pinene, carene and cineole) in yeast. Genes encoding these synthases will be inserted into yeast gateway vectors as well as the biobrick plasmid PSB1C3.


Metabolic Genes

Our goal is to express metabolic enzymes (specifically, erg20-2, Idi1 and Hmg2) that optimize the mevalonate pathway in yeast for monoterpene production.


Production of Monoterpenes in Yeast

From an industrial perspective, this yeast will be cultured and harvested for monoterpenes, which will be analysed by gas chromatography mass spectrometry (GC-MS). Yeast is a highly utilized organism in industry (e.g. breweries and also the recently established Amyris biotech company that uses yeast to produce anti-Malarial therapeutics amongst other valuable compounds). We envision that monoterpene-producing yeast will not only be industrially profitable, but also help to alleviate the unsustainable consumption of nature's resources in the quest for biofuels, pharmaceuticals, flavours/fragrances etc.

From an environmental perspective, this yeast will be co-cultured with the bluestain fungus to determine if monoterpene-producing yeast is able to inhibit the growth of the fungus. This yeast is also a biological system that can be screened for potential targets against the fungus. For instance, since the yeast is actively producing monoterpenes, it is possible to look for yeast that have particular mutations that cause increased intolerance against monoterpenes. These mutations will reveal potential strategies against the fungus.

Characterization details here!



Computational Modeling

Mountain Pine Beetle Epidemic

A mathematical model that can predict the dynamics of the mountain pine beetle populations in B.C. under the influence of our synthetic product. This model can be coupled with the strategic planning for future conservation initiatives.

Monoterpene Production in Yeast

A model that can assay the production of monoterpenes in our genetically engineered yeast cells given that they contain the metabolic genes IDI1, K6R-hmg2, erg20-2 and a monoterpene synthase.

Synthase Structure

A model that can determine monoterpene synthase product specificity and enzymatic activity based on amino acid sequence.

Modeling details here!



Human Practices

Mentorship

  1. Outreach at Science World: Start a high school iGEM team and mentor students participating in the Future Leaders in Science program
  2. Initiate collaboration with other iGEM teams to compile an iGEM dictionary for synthetic biology and iGEM specific terms

Public Perceptions

  1. Inform hundreds in the UBC student community about our project and iGEM at Imagine Day Orientation
  2. Tracking changes in public perception by asking for and collating definitions of synthetic biology each year at UBC

Synthetic Biology in the Wild

  1. Conduct interviews with experts in relevant fields to review the implications of introducing synthetic organisms into the environment


Multimedia and outreach details here!

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