Team:British Columbia/Notebook/Week 19

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Team: British Columbia - 2011.igem.org


Week 19: Oct 9-15

Mountain Pine Beetle and Saccharomyces cerevisiae Co-culturing

Ubcigembeetleexp1.jpg

We ran the beetle transfer experiments not only with the mountain pine beetle, but also with the white pine weevil. Here we are getting the beetles from a generous benefactor.

Ubcigembeetleexp3.jpg

The White Pine Weevil.

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Next came the fun part! We followed the beetle transfer protocol (see 'protocols'), transferring the beetle to the yeast plates, followed by placing them on a clean plate. After growing the plates (there was a lot of contamination), we analyzed the resultant yeast colonies to check for GFP and clonat resistance.

Ubcigembeetleexp6.jpg

The deadly Mountain Pine Beetle!

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The MPB on a yeast plate.

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The beetles appeared to try to get the yeast off their backs by rolling around.

Ubcigembeetleexp2.jpg

We noticed a brown spot on one of the beetles, and wanted to get a closer look. It turned out to be nothing, but then we heard we might see a mite! Alina searched long and hard for a mite, and found this beauty on one of the beetles.

Dung beetles

Ubcigembeetleexp4.jpg





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