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Team:British Columbia/Notebook/Week 11
From 2011.igem.org
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Lab Meeting - August 15
beta-Pinene
Still working on getting the original synthase onto a yeast plasmid containing GPD promoter, she re-dos the ligations using a 6:1 ratio instead of 3:1. She then transforms the product into E.coli. Vicki also digests and ligates the original synthase and the yeast plasmid (GAL) together and transforms that into E.coli.
Characterization: Limonene Synthase - Part BBa_K118025
Vicki digested and ligated following samples:
- limonene synthase + yeast plasmid with GPD promoter
- limonene synthase + yeast plasmid with GAL promoter
She then transformed the products into E.coli cells. Waiting for results...
