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Team:British Columbia/Notebook/Week 11
From 2011.igem.org
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==beta-Pinene== | ==beta-Pinene== | ||
| + | Still working on getting the original synthase onto a yeast plasmid containing GPD promoter, she re-dos the ligations using a 6:1 ratio instead of 3:1. She then transforms the product into <i>E.coli</i>. | ||
| + | Vicki also digests and ligates the original synthase and the yeast plasmid (GAL) together and transforms that into <i>E.coli</i>. | ||
| - | == | + | ==Characterization: Limonene Synthase - Part BBa_K118025== |
| + | Vicki digested and ligated following samples: | ||
| + | #limonene synthase + yeast plasmid with GPD promoter | ||
| + | #limonene synthase + yeast plasmid with GAL promoter | ||
| + | She then transformed the products into <i>E.coli</i> cells. Waiting for results... | ||
Revision as of 02:11, 15 August 2011
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Lab Meeting - August 15
beta-Pinene
Still working on getting the original synthase onto a yeast plasmid containing GPD promoter, she re-dos the ligations using a 6:1 ratio instead of 3:1. She then transforms the product into E.coli. Vicki also digests and ligates the original synthase and the yeast plasmid (GAL) together and transforms that into E.coli.
Characterization: Limonene Synthase - Part BBa_K118025
Vicki digested and ligated following samples:
- limonene synthase + yeast plasmid with GPD promoter
- limonene synthase + yeast plasmid with GAL promoter
She then transformed the products into E.coli cells. Waiting for results...
