Team:SJTU-BioX-Shanghai/Project/Subproject1/Results

Rare-Codon Switch

Results

Action: Combinations of Modulators and Reporters

Select a Modulator and a Reporter, then click to see results!

tRNA Modulator System

• System Analysis

tRNA Modulator + Reporter for Qualitative Analysis

Action: when rare tRNA^Arg is over expressed, the amount of tRNA^Arg in the tRNA pool is largely increased, which facilitates ribosome to get through the tandem of AGG codons in the RFP's mRNA.

tRNA Modulator + Reporter for Quantitative Analysis

Action: when rare tRNA^Arg is over expressed, the amount of tRNA^Arg in the tRNA pool is largely increased, which facilitates ribosome to get through the tandem of AGG codons in the modified luciferase's mRNA.

• Conclusion

We have analyzed the three potential factors influencing Rare-Codon Switch and have fully characterized our device through working curves, demonstrating the function of this device as a regulating tool for protein biosynthesis. We have also identified influential factors other than the three proposed above.

aaRS Modulator System

• System Analysis

aaRS Modulator + Reporter for Qualitative Analysis

Action: When TDRS is expressed under IPTG induction, it can charge tRNA^Asp-AGG with Asp. tRNA^Asp-AGG then recognizes the rare codon AGG on RFP's mRNA and add Asp to the polypeptide chain. RFP is expressed.

aaRS Modulator + Reporter for Quantitative analysis

Action: When TDRS is expressed under IPTG induction, it can charge tRNA^Asp-AGG with Asp. tRNA^Asp-AGG then recognizes the rare codon AGG on the modified luciferase's mRNA and add Asp to the polypeptide chain. Modified luciferase is expressed.

• Conclusion

We have designed modified AspRS and tested its aminoacylation ability, ensuring the function of our Rare-Codon Switch.