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Team:SouthBend-Mishawaka-HS-2/Notebook
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You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.
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Notebook
05/19/11 Agenda
(1) Obtained a vial of 2mL of TOP-10 electro competant E. coli cells 3-16-11 (44-0003/793762)
(2) Added 50 microliters of BIO-RAD transformation solution (Control number 31000008916 recd 1-17-11 GT)
(3) Added 2 microliters of DNA from Registry plate 2 well 5b
(4) Incubate on ice 5 minutes.
(5) Heat shocked 40 degrees C for 50 seconds
(6) Incubuated 4 degrees C for 50 seconds
(7) Added 1mL LB 5-17-11 D.G.
(8) Plate 100 microliters and 25 microliters of transformed cells on LB-AMP (5-19-11 "who")
