30.07.11

https://static.igem.org/mediawiki/igem.org/a/a2/30%2C07%2C2011.pdf

31.07.11

PLASMID ISOLATION
-K(J04450)+Shuttle(K090403) 1/2/3
-1002+1006+K

  GLYCEROL STOCK
-K+shuttle(K090403)
-1006
-1001
-1001+1006+K
-1002+1006+K
-1004+1006+K
-K143053+1006+K

DIGASTION,LIGASTION (with pst and ecoR1)
-1001+1005/shuttle
-1002+1005/shuttle
-1001+1006/shuttle
-1002+1006/shuttle
-K143053+1006/shuttle

  TRANSFORMATION
-1001+1005/shuttle
-1002+1005/shuttle
-1001+1006/shuttle
-1002+1006/shuttle
-K143053+1006/shuttle

  LIQUID CULTURE
-1002
-1003
-1004
-1005
we prepared those plasmids with amphiciline.

  -1002+K
-1003+K
-1004+K
-1005+K
-1001+1005+K (only plasmid isolation)
-1002+1005+k
-1004+1005+K
-1003+1006+K
-J04500+1006+K
we prepared those plasmids with chloramphenicol

https://static.igem.org/mediawiki/igem.org/7/79/31%2C07%2C2011.pdf

AUGUST 2011

01.08.11

https://static.igem.org/mediawiki/igem.org/8/8e/01%2C08%2C2011.pdf