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Team:UNIST Korea/project/future work
From 2011.igem.org
Future work & Application
In the future our first effort will be to optimize the lysis module such that the Dpn enzymes are efficiently exploited by the synthetic microbes to eradicate its genetic material in a non-native environment.
On top of this, UNIST team will also introduce more controlled system into E.coli so that it can be more safe to the environment and easy to be handled in the laboratory.
We would like to introduce the following components into our Chop. coli in order to achieve our goal
Our team will also introduce a fourth sensing module to the Chop. coli using the quorum sensing system. In case of fermentor E. coli will experience its native original quorum sensing molecule, AI2 UNIST team is going to make that engineer E.coli to recognize different quorum sensing molecules such as AHL to differentiate between the fermentor and the real world. This system will also help achieve an effective and tight control of lysis device.
Thirdly, We have been trying to concentrate on more accurate control of gene expressions and effective decrease of leaky levels in our circuits by designing the temperature-dependent mRNA secondary structure, but since it might not be working well completely for now probably this is expected to be accomplished with more clear experimental data, which will play a key role in the future construct.
Fourthly, We also have been focusing on making good lysis module introduced in our E.coli using either Holin system or Dpn system. Unfortunately, the result was not that clear as much as we expected. Thus, for the greater progress, UNIST iGEM team is determined to work on the experiment much about lysis system in the future.
Lastly, additional productive circuit may join to the whole construct in the long future in order that E.coli can produce something genuinely valuable to the other things for applications because they may not be much worthy if they have nothing to produce anything although lots of controlled systems exist in the whole construct. For instance, as the additional productive systems for real applications, there might be systems containing the genes which are going to be much beneficial to the environment, or human health & well being, etc.
