Team:SJTU-BioX-Shanghai/Parts/Data Page

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    Data Page

    Rare-Codon Switch

    Rare-Codon Switch: control with rare tRNA amount

    11SJTU rare 01.jpg
    • Data for our favorite new parts
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567001 Main Page] - BBa_K567001 lacI -Ptrc-tRNAArg: The tRNAArg(drived from E.coli ArgW operon) is under the control of trc promoter, induced by IPTG.
    • Data for Pre-existing Parts
    1. [http://partsregistry.org/Part:BBa_I712019:Experience Experience] - BBa_I712019 Firefly luciferase (Anja Korenčič 2007)
    • We've Also Characterized the Following Part
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567002 Main Page] - BBa_K567002 sulA promoter-tRNAArg :The tRNAArg is under the control of sulA promoter induced by UV.



    Rare-Codon Switch: control with aaRS

    11SJTU rare 03.jpg
    • Data for our favorite new parts
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567011 Main Page] - BBa_K567011 PT7-TDRS :Aspartyl aminoacyl tRNA synthetase without anticodon recognition domain under the control of T7 promoter and lac operator.
    2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567012 Main Page] - BBa_K567012 tRNAAsp-AGG : The tRNAAsp-AGG with its anticodon mutated to CCU(base pairing rare codon AGG) and under the control of aspV promoter.
    • Data for Pre-existing Parts
    1. [http://partsregistry.org/Part:BBa_I13521:Experience Experience] - BBa_I13521 Ptet mRFP (Endy Lab 2005)
    • We've Also Characterized the Following Part
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567017 Main Page] - BBa_K567017 PT7-RFP-6AGG



    Rare-Codon Switch: control with rare codon number, locations and tandem copies

    11SJTU rare 12.jpg
    • Data for our favorite new parts
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567001 Main Page] - BBa_K567001 lacI -Ptrc-tRNAArg: The tRNAArg(drived from E.coli ArgW operon) is under the control of trc promoter, induced by IPTG.
    2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567004 Main Page] - PT7/Pbla-Luc-nAGG Date for control with rare codon number
    3. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567021 Main Page] -PT7-Luc-2x4AGG(111bp) Date for control with rare codon number, locations and tandem copies



    Stop-Codon Switch

    11SJTU stop 01.jpg
    • Data for our favorite new parts
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567011 Main Page] - BBa_K567011 PT7-TDRS :Aspartyl aminoacyl tRNA synthetase without anticodon recognition domain under the control of T7 promoter and lac operator.
    2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567013 Main Page] - BBa_K567013 tRNAAsp-TAG : The tRNAAsp-TAG with its anticodon mutated to CUA (base pairing stop codon UAG) and under the control of aspV promoter.
    • We've Also Characterized the Following Part
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567003 Main Page] - BBa_K567003 Pbla-Luc-TAG
    • We've Also Made the Following Part
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567018 Main Page] - BBa_K567018 PT7-GFP-TAG-RFP



    Initial-Codon Switch

    • Data for our favorite new parts
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567014 Main Page] - BBa_K567014 PT7-metGM : T7 promoter-metG(mutated). This biobrick is constructed by putting the mutated metG (Met-RS) under the control of T7 promoter and lac operator.
    2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567015 Main Page] - BBa_K567015 PT7-metGN :T7 promoter-metG(truncated). This biobrick is constructed by putting the truncated metG (Met-RS) under the control of T7 promoter and lac operator.
    3. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567016 Main Page] - BBa_K567016 metY-CGA :This biobrick is constructed by mutating the anticodon of tRNAmet to TCG (base pairing codon CGA).
    • We've Also Characterized the Following Part
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567020 Main Page] - BBa_K567020 KanR-CGA


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