Team:British Columbia/Accomplishments

Project Accomplishments

Contents 1 Wet Laboratory Results 2 1. In vitro assay production of monoterpene in bacteria 3 2. Diterpene production in yeast 4 3. Mountain Pine Beetle & Yeast Co-culture 5 2. Blue Stain Fungi & Yeast Co-culture 6 Modeling Results

Wet Laboratory Results

1. In vitro assay production of monoterpene in bacteria

Geranyl Pyrophosphate (GPP) Assay

Alpha-beta pinene and Beta-pinene synthases were The purified enzymes were assayed in vitro with GPP and sent for GC-MS, which confirmed alpha- and beta-pinene as products.

2. Diterpene production in yeast

3. Mountain Pine Beetle & Yeast Co-culture

2. Blue Stain Fungi & Yeast Co-culture

Modeling Results

Monoterpene Production

We simulated the expansion of the MPB population from year 2011 to 2020 using the estimates obtained from the clustering analysis. For cost estimation and prediction of emergence of subpopulations, refer to our Model Methodology above.