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Post-Regionals

Journal

• Defining Objectives
• Techniques Workshop
• Bioinformatics & qRT-PCR
• Sensory Element Fishing
• Electrochem - CPR Oxidation
• Electrochem - Reporter Gene
• Pseudomonas Tools
• Microalgae Tools
• NA Viability Assays
• May - July Group Journal

Protocols

Safety

Here is a list of all the procedures we used this summer. Each contains a description and list of materials required.

Microalgae Protocols

• Preparation of Algal f2 Media
• Glass Bead Algal Transformation
• Chloroplast Isolation
• Nuclear DNA Extraction from Algae

Pseudomonas Biotinylation Techniques

• Plasmid Isolation (from Pseudomonas spp.)
• Genomic DNA Isolation (from Pseudomonas spp.)
• Biotinylation/Immunoprecipitation of Naphthenic Acids
• Conjugation of E. coli plasmids to Pseudomonas spp..

Electrochemical Techniques

• Electrode Plating
• Buffer Preparation
• Electrical Settings
• Testing For Oxidation

General Protocols

• Plasmid Isolation (from E. coli)
• RNA Extraction
• cDNA Synthesis
• Quantitative Real Time PCR
• Testing the pBAD-lacZ Construct
• Taq PCR Protocol
• PCR Purification
• Gel Extraction
• Testing the lacI-lacZ I732901 Gene
• Bacterial Transformation
• Rehydration of Registry DNA
• Construction Techniques
• Agarose Gel Electrophresis
• LB Agar Plates
• Overnight Cultures
• Preparing Chemically Competent Cells (E. coli)
• Preparing Glycerol Stocks (E. coli)