Team:Calgary/Notebook/Protocols
From 2011.igem.org
![](https://static.igem.org/mediawiki/2011/c/c1/UCalgary_CircleHome.png)
![](https://static.igem.org/mediawiki/2011/d/d4/UCalgary_CircleTeam.png)
![](https://static.igem.org/mediawiki/2011/7/7d/UCalgary_CircleProject.png)
![](https://static.igem.org/mediawiki/2011/8/8d/UCalgary_CircleParts.png)
![](https://static.igem.org/mediawiki/2011/0/03/UCalgary_CircleNotebook.png)
![](https://static.igem.org/mediawiki/2011/8/84/UCalgary_CircleOutreach.png)
![](https://static.igem.org/mediawiki/2011/3/3c/UCalgary_CircleSponsors.png)
![](https://static.igem.org/mediawiki/2011/8/85/UCalgary_CircleiGEM.png)
![](https://static.igem.org/mediawiki/2011/4/42/UCalgary_TitleBar.png)
Protocols
![](https://static.igem.org/mediawiki/2011/e/ed/UCalgary_BodyBoxTop.png)
Here is a list of all the procedures we used this summer. Each contains a description and list of materials required.
- Preparation of Algal f2 Media
- Glass Bead Algal Transformation
- Chloroplast Isolation
- Nuclear DNA Extraction from Algae
- Plasmid Isolation (from Pseudomonas spp.)
- Genomic DNA Isolation (from Pseudomonas spp.)
- Biotinylation/Immunoprecipitation of Naphthenic Acids
- Conjugation of E. coli plasmids to Pseudomonas spp..
- Electrode Plating
- Buffer Preparation
- Electrical Settings
- Testing For Oxidation
- Plasmid Isolation (from E. coli)
- RNA Extraction
- cDNA Synthesis
- Quantitative Real Time PCR
- Testing the pBAD-lacZ Construct
- Taq PCR Protocol
- PCR Purification
- Gel Extraction
- Testing the lacI-lacZ I732901 Gene
- Bacterial Transformation
- Rehydration of Registry DNA
- Construction Techniques
- Agarose Gel Electrophresis
- LB Agar Plates
- Overnight Cultures
- Preparing Chemically Competent Cells (E. coli)
- Preparing Glycerol Stocks (E. coli)
Microalgae Protocols
Pseudomonas Biotinylation Techniques
Electrochemical Techniques
General Protocols
![](https://static.igem.org/mediawiki/2011/c/cb/UCalgary_BodyBoxBottom.png)