Team:Calgary/Notebook/Protocols/Process18
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Overnight Cultures
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Reagents and Materials (per culture prepared):
- 10mL culture tube. Use 16mm x 160mm or 16mm x 125mm
- 5 mL LB
- 5 uL 1000X antibiotics
- Single colonies on a plate (best not to start an over night from a glycerol stock)
Protocol
- Add 5mL sterile/autoclaved LB in a 10mL culture tube.
- Pipet 5μL of 1000X antibiotic into the LB.
- Select a single colony using a sterile toothpick or pipette tip.
- Place toothpick or pipette tip in the culture tube and stir.
- Remove toothpick, or in the case of a pipette tip, leave in the tube.
- Place culture tube in incubator at 37°C overnight shaking vigorously (250 RPM)
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