Team:Calgary/Notebook/Protocols/Process28
From 2011.igem.org
![](https://static.igem.org/mediawiki/2011/c/c1/UCalgary_CircleHome.png)
![](https://static.igem.org/mediawiki/2011/d/d4/UCalgary_CircleTeam.png)
![](https://static.igem.org/mediawiki/2011/7/7d/UCalgary_CircleProject.png)
![](https://static.igem.org/mediawiki/2011/8/8d/UCalgary_CircleParts.png)
![](https://static.igem.org/mediawiki/2011/0/03/UCalgary_CircleNotebook.png)
![](https://static.igem.org/mediawiki/2011/8/84/UCalgary_CircleOutreach.png)
![](https://static.igem.org/mediawiki/2011/3/3c/UCalgary_CircleSponsors.png)
![](https://static.igem.org/mediawiki/2011/8/85/UCalgary_CircleiGEM.png)
![](https://static.igem.org/mediawiki/2011/4/42/UCalgary_TitleBar.png)
Testing For Oxidation
![](https://static.igem.org/mediawiki/2011/e/ed/UCalgary_BodyBoxTop.png)
For this procedure you will need:
- Freshly prepared buffer solution
- Solution to be measured
- Freshly plated electrode
- Potentiostat with electrode adapter
- AfterMath installed on a computer
This procedure will show oxidization of a compound and can generate standard curves. A picture of the potentiostat and connected electrode is shown at the bottom of the page.
- Place the plated electrode into the adapter (the use of a blank spacer may be needed)
- Place the connected electrode into the buffer solution and take a blank reading through AfterMath
- Add the solution to be measured and test for oxidation through AfterMath
- Dispose of all solutions and electrodes according to the policies in your facility
![](https://static.igem.org/mediawiki/2011/5/5b/UofC_ElecSetup.jpg)
![](https://static.igem.org/mediawiki/2011/c/cb/UCalgary_BodyBoxBottom.png)