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Team:Calgary/Notebook/Protocols/Process27
From 2011.igem.org
Notebook
Post-Regionals
Journal
- Defining Objectives
- Techniques Workshop
- Bioinformatics & qRT-PCR
- Sensory Element Fishing
- Electrochem - CPR Oxidation
- Electrochem - Reporter Gene
- Pseudomonas Tools
- Microalgae Tools
- NA Viability Assays
- May - July Group Journal
Protocols
Safety
Electrical Settings
For the generation of a standard curve we used a Pin Instruments WaveNow potentiostat. The accompanying software, AfterMath, was used to record the data. Below is a screenshot of the settings used during the experiment.
This range will accomodate the oxidation of chlorophenol red and give enough room to generate a sufficient standard curve. The 6 segments means that the data generated is in triplicate. Varying the sweep rate will alter the resolution of the graph, with smaller sweep rates causing higher resolutions.
