Team:Osaka/week12

October 16 (Sun)

Reginal Jamboree in HKUST

October 17 (Mon)

October 18 (Tue)

1. Restriction digests
2. Ligation
   • 116(T):105(upstream)+1-23L(downstream)+1-7A(vector)
   • 117(T):106(upstream)+1-23L(downstream)+1-7A(vector)
   • 118(T):107(upstream)+1-23L(downstream)+1-7A(vector)
   • 119(T):108(upstream)+1-23L(downstream)+1-7A(vector)
3. Transformation of Ligation products

October 19 (Wed)

1. To liquid culture:116,117,118,119
2. Plating
3. Ligation
   • 120(T):105(upstream)+1-23L(downstream)+1-5A(vector)
   • 121(T):106(upstream)+1-23L(downstream)+1-5A(vector)
   • 122(T):107(upstream)+1-23L(downstream)+1-5A(vector)
   • 123(T):108(upstream)+1-23L(downstream)+1-5A(vector)
4. Transformation of ligation products

October 20 (Thu)

1. Miniprep of yesterday's culture
2. Restriction digests of miniprepped parts
3. Electrophoresis of digests
   • 118 x
4. Ligation
   • 124(C):116(upstream)+117(downstream)+1-3A(vector)
   • 125(C):116(upstream)+119(downstream)+1-3A(vector)
   • 126(C):117(upstream)+119(downstream)+1-3A(vector)
5. To liquid culture:118
6. Transformation of ligation products

October 21 (Fri)

1. Miniprep of yesterday's culture
2. Restriction digests of miniprepped parts
3. Electrophoresis of digests
4. Ligation
   • 127(C):116(upstream)+118(downstream)+1-3A(vector)
   • 128(C):117(upstream)+118(downstream)+1-3A(vector)
   • 129(C):118(upstream)+119(downstream)+1-3A(vector)
5. To liquid culture:124,125,126
6. Transformation of ligation products
7. Plating (Tolerance assay)

October 22 (Sat)

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