Team:Osaka/week12

October 16 (Sun)

Reginal Jamboree in HKUST

October 17 (Mon)

October 18 (Tue)

1. Restriction digests
2. Ligation
   • 116(T):105(upstream)+1-23L(downstream)+1-7A(vector)
   • 117(T):106(upstream)+1-23L(downstream)+1-7A(vector)
   • 118(T):107(upstream)+1-23L(downstream)+1-7A(vector)
   • 119(T):108(upstream)+1-23L(downstream)+1-7A(vector)
3. Transformation of Ligation products

October 19 (Wed)

1. To liquid culture:116,117,118,119
2. Plating
3. Ligation
   • 120(T):105(upstream)+1-23L(downstream)+1-5A(vector)
   • 121(T):106(upstream)+1-23L(downstream)+1-5A(vector)
   • 122(T):107(upstream)+1-23L(downstream)+1-5A(vector)
   • 123(T):108(upstream)+1-23L(downstream)+1-5A(vector)
4. Transformation of ligation products

October 20 (Thu)

1. Miniprep of yesterday's culture
2. Restriction digests of miniprepped parts
3. Electrophoresis of digests
   • 118 x
4. Ligation
   • 124(C):116(upstream)+117(downstream)+1-3A(vector)
   • 125(C):116(upstream)+119(downstream)+1-3A(vector)
   • 126(C):117(upstream)+119(downstream)+1-3A(vector)
5. To liquid culture:118
6. Transformation of ligation products

October 21 (Fri)

1. Miniprep of yesterday's culture
2. Restriction digests of miniprepped parts
3. Electrophoresis of digests
4. Ligation
   • 127(C):116(upstream)+118(downstream)+1-3A(vector)
   • 128(C):117(upstream)+118(downstream)+1-3A(vector)
   • 129(C):118(upstream)+119(downstream)+1-3A(vector)
5. To liquid culture:124,125,126
6. Transformation of ligation products
7. Plating (Tolerance assay)

October 22 (Sat)

1. Colony counts (Tolerance assay)
2. Miniprep of yesterday's culture
3. Restriction digests of miniprepped parts
4. Electrophoresis of digests
5. Gel extraction
6. Ligation
   • 130(C):124(upstream)+118(downstream)+1-3A(vector)
   • 131(C):124(upstream)+119(downstream)+1-3A(vector)
   • 132(C):126(upstream)+118(downstream)+1-3A(vector)
   • 133(C):01+1-3A(vector)
   • 134(C):02+1-3A(vector)
   • 135(C):1-12M+1-3A(vector)
   • 136(C):01(upstream)+119(downstream)+1-3A(vector)
   • 137(C):02(upstream)+119(downstream)+1-3A(vector)
   • 138(C):116+1-3A(vector)
   • 139(C):117+1-3A(vector)
   • 140(C):118+1-3A(vector)
   • 141(C):119+1-3A(vector)
7. Transformation of products

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