Team:Osaka/week8

September 18 (Sun)

1. Miniprep of yesterday's culture
2. Restriction digests of minipreppped parts
3. Gel electrophoresis of digests
4. To liquid culture:03,105,106,107,108,109,110,111,112

September 19 (Mon)

1. Miniprep of yesterday's culture
2. Restriction digests of minipreppped parts
3. Gel electrophoresis of digests
   • ok
4. Ligation
   • 113(T):105(upstream)+110(downstream)+1-7A(vector)
   • 114(T):111(upstream)+112(downstream)+1-7A(vector)
5. Transformation of ligation products
6. Plating (cell survival)

September 20 (Tue)

1. To liquid culture:113,114
2. colony check (cell survival)

September 21 (Wed)

1. Miniprep of yesterday's culture
2. Restriction digests of minipreppped parts
3. Gel electrophoresis of digests
   • ok
4. Ligation
   • 115(A):113(upstream)+114(downstream)+1-1G(vector)
5. Transformation of ligation products
6. colony check (cell survival)
7. Plating (cell survival)

September 22 (Thu)

1. Colony check
2. Plating
3. To liquid culture

September 23 (Fri)

1. Miniprep of yesterday's culture
2. Restriction digests of minipreppped parts
3. Gel electrophoresis of digests
   • ok
4. Colony check

September 24 (Sat)

1. Preparation of LB plates
2. Plating

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