Team:Osaka/week13

October 23 (Sun)

1. Miniprep of yesterday's culture
2. Restriction digests of miniprepped parts
3. Electrophoresis of digests
4. Gel extraction
5. Ligation
   • 142(C):126(upstream)+118(downstream)+1-3A(vector)
6. To liquid culture:130,131,132,133,134,138,139,140,141
7. Transformation of ligation products

October 24 (Mon)

1. Miniprep of yesterday's culture
2. Restriction digests of miniprepped parts
3. Electrophoresis of digests
   • 131,133,134 x
4. Gel extraction
5. Ligation
   • 143(C):132(upstream)+118(downstream)+1-3A(vector)
6. To liquid culture:131,133,134,142
7. Plating (Tolerance assay)
8. Transformation of ligation products

October 25 (Tue)

1. Miniprep of yesterday's culture
2. Restriction digests of miniprepped parts
3. Electrophoresis of digests
4. To liquid culture:130,131,132,133,134,135,136,137,142
5. Colony counts (Tolerance assay)
6. Plating (Tolerance assay)

October 26 (Wed)

1. Miniprep of yesterday's culture
2. Restriction digests of miniprepped parts
3. Electrophoresis of digests
4. To liquid culture:131,133,134,137
5. Colony counts (Tolerance assay)
6. Plating (Tolerance assay)

October 27 (Thu)

1. Miniprep of yesterday's culture
2. Restriction digests of miniprepped parts
3. Electrophoresis of digests
4. Transformation
5. Plating (Tolerance assay)
6. Colony counts (Tolerance assay)

October 28 (Fri)

1. Colony counts (Tolerance assay)

October 29 (Sat)

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