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User contributions
From 2011.igem.org
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- 01:34, 29 September 2011 (diff | hist) Miniprep Quiagen Kit
- 01:29, 29 September 2011 (diff | hist) N Gel DNA extraction (Created page with "[https://2011.igem.org/Team:Panama/Protocols/Wetlab '''Back''']")
- 01:29, 29 September 2011 (diff | hist) N NZY+ Recipe (Created page with "[https://2011.igem.org/Team:Panama/Protocols/Wetlab '''Back''']")
- 01:29, 29 September 2011 (diff | hist) N Primer aliquots (Created page with "[https://2011.igem.org/Team:Panama/Protocols/Wetlab '''Back''']")
- 01:28, 29 September 2011 (diff | hist) N Agar plates (Created page with "[https://2011.igem.org/Team:Panama/Protocols/Wetlab '''Back''']")
- 01:28, 29 September 2011 (diff | hist) PCR
- 01:27, 29 September 2011 (diff | hist) PCR purification
- 01:27, 29 September 2011 (diff | hist) N Miniprep "Dirty" (Created page with "[https://2011.igem.org/Team:Panama/Protocols/Wetlab '''Back''']") (top)
- 01:26, 29 September 2011 (diff | hist) N Miniprep Quiagen Kit (Created page with "[https://2011.igem.org/Team:Panama/Protocols/Wetlab '''Back''']")
- 01:25, 29 September 2011 (diff | hist) Mutagenesis PCR (top)
- 01:24, 29 September 2011 (diff | hist) N LB media recipe (Created page with "[https://2011.igem.org/Team:Panama/Protocols/Wetlab '''Back''']")
- 01:24, 29 September 2011 (diff | hist) N Preparing chemically competent cells (Created page with "[https://2011.igem.org/Team:Panama/Protocols/Wetlab '''Back''']")
- 01:24, 29 September 2011 (diff | hist) N Pgem Cloning (Created page with "[https://2011.igem.org/Team:Panama/Protocols/Wetlab '''Back''']")
- 01:23, 29 September 2011 (diff | hist) N Colony PCR (Created page with "[https://2011.igem.org/Team:Panama/Protocols/Wetlab '''Back''']")
- 01:15, 29 September 2011 (diff | hist) Agarose gel electrophoresis (top)
- 01:15, 29 September 2011 (diff | hist) N Mutagenesis transformation (Created page with "[http://www.genomics.agilent.com/files/Manual/210514.pdf '''Quiklightning Multi-site directed mutagenesis kit.'''] [https://2011.igem.org/Team:Panama/Protocols/Wetlab '''Back''']")
- 01:12, 29 September 2011 (diff | hist) Mutagenesis PCR
- 01:04, 29 September 2011 (diff | hist) N Mutagenesis PCR (Created page with "Mutagenesis PCR was done using the protocol established by agilent's [http://www.genomics.agilent.com/files/Manual/210514.pdf '''quicklightning Multi-site directed mutagenesis ki...")
- 00:52, 29 September 2011 (diff | hist) PCR purification (Blanked the page)
- 00:52, 29 September 2011 (diff | hist) N PCR purification (Created page with "fjdskl")
- 00:45, 29 September 2011 (diff | hist) Transformation (top)
- 00:43, 29 September 2011 (diff | hist) Transformation
- 00:35, 29 September 2011 (diff | hist) N File:Comic strip.jpg
- 00:29, 29 September 2011 (diff | hist) Transformation
- 00:27, 29 September 2011 (diff | hist) N File:Comic strip.gif (top)
- 00:06, 29 September 2011 (diff | hist) Team:Panama/Protocols/Wetlab
- 23:59, 28 September 2011 (diff | hist) N PCR (Created page with "'''Materials:''' DNA template Invitrogen platinium blue supermix Forward primer Reverse Primer Molecular grade H2O '''Procedure:''' 1.")
- 20:49, 28 September 2011 (diff | hist) N "Overnight" Cell Culture (Created page with "'''Materials:''' Bacterial sample LB broth 15 ml centrifuge tube '''Procedure:''' 1. Add 2ml of sterile LB broth into a 15ml centrifuge tube. 2. Inoculate the tube with 50u...") (top)
- 20:47, 28 September 2011 (diff | hist) Team:Panama/Protocols/Wetlab
- 20:47, 28 September 2011 (diff | hist) Cell Culture (top)
- 20:47, 28 September 2011 (diff | hist) N Cell Culture (Created page with "'''Materials: ''' Bacterial sample LB broth 15 ml centrifuge tube '''Procedure:''' 1. Add 2ml of sterile LB broth into a 15ml centrifuge tube. 2. Inoculate the tube with 50u...")
- 20:40, 28 September 2011 (diff | hist) Chloramphenicol stock (top)
- 20:40, 28 September 2011 (diff | hist) Chloramphenicol stock
- 20:39, 28 September 2011 (diff | hist) N Chloramphenicol stock (Created page with "Materials: Chloramphenicol powder ddH2O sterile syringe syringe filter sterile 15ml centrifuge tube Procedure: 1. Weigh the appropriate amount of antibiotic powder for the amo...")
- 20:36, 28 September 2011 (diff | hist) Team:Panama/Protocols/Wetlab
- 20:35, 28 September 2011 (diff | hist) Ampicillin stock (top)
- 20:35, 28 September 2011 (diff | hist) Ampicillin stock
- 20:22, 28 September 2011 (diff | hist) N Glycerol Stock (Created page with "'''Materials:''' Cell culture 40% glycerol solution '''Procedure:''' 1. Grow an "overnight" cell culture of the desired sample. 2. Add 1ml of "overnight" culture into a 2ml ...") (top)
- 07:48, 28 September 2011 (diff | hist) Ampicillin stock
- 07:46, 28 September 2011 (diff | hist) N Ampicillin stock (Created page with "Materials: Ampicillin powder ddH2O [https://2011.igem.org/Team:Panama/Protocols/Wetlab '''Back''']")
- 07:41, 28 September 2011 (diff | hist) Agarose gel electrophoresis
- 07:40, 28 September 2011 (diff | hist) Agarose gel electrophoresis
- 07:29, 28 September 2011 (diff | hist) Agarose gel electrophoresis
- 07:28, 28 September 2011 (diff | hist) Agarose gel electrophoresis
- 07:27, 28 September 2011 (diff | hist) Agarose gel electrophoresis
- 07:26, 28 September 2011 (diff | hist) Team:Panama/Protocols/Wetlab
- 07:25, 28 September 2011 (diff | hist) Team:Panama/Protocols/Wetlab
- 07:23, 28 September 2011 (diff | hist) N Agarose gel electrophoresis (Created page with "'''Materials:''' DNA template Agarose TAE/TBE buffer 6X loading buffer '''Procedure:''' 1. Prepare your gel according to the size of your DNA template. (''a 1% gel works f...")
- 06:58, 28 September 2011 (diff | hist) Team:Panama/Protocols/Wetlab
- 00:18, 22 September 2011 (diff | hist) Team:Panama/Protocols (top)
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