"Overnight" Cell Culture

From 2011.igem.org

Materials:

Bacterial sample

LB broth

15 ml centrifuge tube

Procedure:

1. Add 2ml of sterile LB broth into a 15ml centrifuge tube.

2. Inoculate the tube with 50ul of liquid bacterial sample or a single colony from an agar plate.

3. Incubate at 37°C from one evening to the next morning. ("overnight")

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