Mutagenesis PCR
From 2011.igem.org
Mutagenesis PCR was done using the protocol established by agilent's [http://www.genomics.agilent.com/files/Manual/210514.pdf Quiklightning Multi-site directed mutagenesis kit.]
Our Mutagenesis Primers were:
1. 5' - CGAATCACGTGCTACAGCGCGCCTACG - 3'
2. 5' - ACCTGCCGCACTGTAGTTTCTCGCGGG - 3'
3. 5' - CTCGACCGAACACCTACAGGGCGACTTCTAC - 3'