Glycerol Stock

From 2011.igem.org

Materials:

Cell culture

40% glycerol solution

Procedure:

1. Grow an "overnight" cell culture of the desired sample.

2. Add 1ml of "overnight" culture into a 2ml microtube or cryovial.

3. Add 1ml of 40% glycerol solution into the same tube.

4. Store at -80°C.( To reanimate simple inoculate 50ul into fresh LB broth with the appropriate antibiotic)

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